Many imaging technologies can visualize cells in vivo, such as ultrasound, optics, MRI and nuclear imaging, and these methods need to correspond to different labeling and detection strategies. Each strategy has its own advantages and disadvantages. OBJECTIVE: To review...
Cellular populations of GCs, oocytes and luteal cells were subclustered into six distinct SCLs (Fig.7a–c). GCs were further segregated into four distinct SCLs that were identified as being part of follicles at different stages of development. These SCLs included preantral, antral, mitotic and a...
Cell-Selective Metabolic Glycan Labeling Based on Ligand-Targeted Liposomes. J. Am. Chem. Soc. 134, 9914–9917 (2012). Article CAS PubMed Google Scholar Fan, X. et al. Cell-type-specific labeling and profiling of glycans in living mice. Nat. Chem. Biol. 18, 625–633 (2022). ...
This unit describes metabolic labeling techniques that provide specific information about the structure, sequence, and distribution of the sugar chains of glycoconjugates. In the Basic Protocol, cells in culture are grown through several population doublings in complete medium supplemented with radiolabeled ...
The purpose of this bibliography is to list all available references on the various aspects of mycoplasma infection of animal cell cultures. Topics covered include: general reviews and textbooks; basic properties of mycoplasmas; incidence, detection and identification of cell culture mycoplasmas; effects...
[46]. Therefore, given the variable results in nonclinical studies and apparent lack of relevance to the clinical situation, ICH S6(R1) recommends that with these types of compounds, the potential hazard would be best addressed through appropriate product labeling and clinical risk management ...
Tissue sections obtained from the embryonic and adult heart of these chimeric mice were analyzed, and patchy areas of labeling were quantified. The presence of “holes,” or nonlabeled areas, in the patches was also determined. The distribution of labeled areas did not change as a function of...
The brain consists of many cell classes yet in vivo electrophysiology recordings are typically unable to identify and monitor their activity in the behaving animal. Here, we employed a systematic approach to link cellular, multi-modal in vitro properties from experiments with in vivo recorded units ...
Next, multiply this number by 104and then by five, since in this example, the cells were diluted 1:5 when labeling with trypan blue: 61.857 cells x 104x 5 = 309.375 x 104 cells/ml = 3.094 x 106cells/ml (rounded up) This is the viable cell density. If seeding fresh plates, use ...
used with thepiggyBacsystem and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a...