The next day, after a washing step, the spheroids stained with CD133 were incubated with a 1:250 goat anti-mouse dilution of the secondary antibody (Alexa Fluor 555, Invitrogen), where the cells labeled with ß-Catenin were incubated with 1:100 goat anti-rabbit IgG (Alexa Fluor 555, ...
middle, the uniform manifold approximation and projection (UMAP) visualization shows the clusters of spots on the ST slide. Each cluster is labeled according to its histological tissue type, with BE referring to benign epithelium. The right panel shows the UMAP clusters of spots overlaid on the ...
Columns and rows are labeled numerically Command bars of add-ins aren't displayed or removed Command can't be used on multiple selections Conditional formatting is set incorrectly with VBA Convert degrees/minutes/seconds angles Convert Excel column numbers Create a dynamic defined range Create a mont...
Genes with adjusted P < 0.05 for each cell-type pair were labeled as significantly differentially expressed. Venny diagram Venny (https://bioinfogp.cnb.csic.es/tools/venny/index.html) was applied to analysis genes which were specially expressed in cambium and primary xylem cells of Jin668...
labeled THP-1 cells was determined[64–66]. Schildberger et al.[35]activated THP-1 and PBMC–monocytes before co-culturing with HUVEC and found that different cytokine profiles of HUVEC can be distinguished due to the amount of cytokine released from those two cell types. The observations led...
In addition, the cells were labeled with red-fluorescent propidium iodide (P1304MP, P3566, P21493) to assess the total cellular DNA content. The cells were analyzed by flow cytometry using 488 nm excitation; the fluorescent...
This is the cell that will contain a comment with the running total. Follow these steps to run the SetComment macro: On the Tools menu, point to Macro, and then click Macros. In the Macro dialog box, click SetComment, and then click Run. ...
The labeled cell preparations were analyzed by flow cytometry. Molted hens had significantly decreased CT4+ peripheral blood T cells on Day 3 in both trials and on Day 10 in one trial. No effects on peripheral blood CT8+ T cells were observed. Splenic CT4+ T cells were decreased on Day...
To this end, non-NB cells were labeled as normal reference cells. The baseline expression in normal cells was then subtracted from both the tumor cells as well as the normal cells, yielding residual expression values. For this calculation, a dynamic threshold for noise filtering was applied (...
Each cell type was labeled with indicated colors. c UMAP plots show the expression levels of indicated marker genes. Colors represent the log2-transformed normalized counts of genes. d Combined UMAPs with each type of innate T cell divided into cell clusters (colored): iNKT (3285 cells, left...