Image deconvolution and movie generation Z-stacks, either single; static or multiple; time-lapse, were acquired by widefield microscopy. Each z-stack consisted of 11 separate z-slices per sample, with a 1 µm step between slices. Static and time-lapse z-stack images were deconvolved using...
Cells communicate with each other through secreting and releasing proteins and vesicles. Many cells can migrate. In this study, we report the discovery of migracytosis, a cell migration-dependent mechanism for releasing cellular contents, and migrasomes,
8. Aside from all of this it's been gym, gym, gym. Work, work, work. For now (this won't last long) our building is full. And of course the owners are jacking up the rents. A lot! 9. Tomorrow I am off to our corporate office for a Leadership panel discussion group for 3 ...
In addition, a longer, horizontally growing root can coil if the tip grows back in on itself (Additional file 4; time lapse movie Additional file 5). We observed that the twisting action was minimized when the root grew alongside a straight object, which it naturally tends to “hug.” ...
Additional file 1: Movie S1. Cell contraction assay. Terminally differentiated SMCs presented in this movie were derived from HuF1-iPSCs. (AVI 3664 kb) Expression of estrogen receptor in hPSC-derived pSMCs Sex hormones exert their effect through interactions with their nuclear receptors. Estrogen ...
Full size image To further quantify the effect of the photoswitch on the RBC mechanics, we perform micropipette aspiration on the RBCs. Micropipette aspiration is a versatile tool to study the biomechanics of living cells and has been used extensively on both soft and rigid cells11,12,72,73,74...
Full size image Additional File 14: Movie 9. TB84 (ppGpp0) growing on LB agar with 0.4% glucose. 200 frames (one frame per two minutes) were compressed into 20 seconds. (MOV 3 MB) This suggests that the changes in cell size in response to YgjD depletion are mediated through the ala...
Show full outline Cited by (685) Figures (15) Show 9 more figures Extras (9) Download all Document S1. Supplemental Experimental Procedures Movie S1. In Vivo 3D Reconstruction of Confocal Images Showing Schwann Cells Migrating along the Vasculature, Related to Figure 3 Movie S2. 3D ...
However, by time 40 min, some of the cells (11 out of 53) detach from the glass coverslip and round-up (Fig 5B, see examples indicated by blue arrows). The time-lapse movie of this experiment revealed that these detached cells were actually undergoing a novel type of membrane blebbing ...
Show full outline Cited by (708) Figures (7) Show 1 more figure Tables (1) Table Extras (11) Download all Video Video S1. Time-Lapse Imaging of Aβ 42 Uptake by Microglia-like Cells Carrying the APOE3 Allele, Related to Figure 4 Video S2. Time-Lapse Imaging of Aβ 42 Uptake ...