Alcohol-free polyethylene foam freezing container Colour Size 1 Unit Request Pricing Catalog # (Select a product) Alcohol-free polyethylene foam freezing container Request Pricing Product Advantages Eliminate the need for isopropanol with alcohol-free cryopreservation Obtain reproducible freezing profiles ...
Controlled rate freezing apparatus or isopropanol chamber 控速冷冻装置或异丙醇室 Liquid nitrogen storage container 液氮贮存容器 For freezing adherent cells, in addition to the above materials, you need: 为了冷冻附着细胞,除了上述材料,你还需要: Balanced salt solution such as Dulbecco’s Phosphate Buffere...
Conventional methods of cell cryopreservation rely on step-down programmable freezers, which up to now have been considered the gold standard, or passive isopropanol (IPA)-filled containers placed in a –80°C freezer. Some methods even call for unstandardized Styrofoam boxes as the “freezing devi...
A major challenge for in vivo delivery of editing enzymes is the lack of vehicles capable of targeting specific cell types. VLPs can package Cas9 RNP complexes produced by over-expressing Cas9 fused to the carboxy-terminal end of the viral Gag polyprotein during VLP production, but cell-selectiv...
Gently freezing cells in slow-freezing containers after calculating cell density and cell viability (aim for >50%–80% cell viability) Timing: 10 min CRITICAL: From this point on, keep cells on ice to maintain viability. Have a Mr. Frosty freezing container filled with isopropanol (or two St...
(HSA, Baxter, Unterschleißheim, Germany) and 10 % dimethyl sulfoxide (DMSO, Charité-Universitätsmedizin Berlin, Germany), rapidly transferred to a cryovial (Nunc, Langenselbold, Germany) and stored in an isopropanol bath freezing container (Nalgene, Rochester, USA) at – 80°C for 24...
The remaining cells were cryopreserved in cryopreservation media (10% dimethyl sulfoxide and 90% FBS), frozen at − 80 °C in an isopropanol jacketed closed container (Nalgene Cryo freezing container), and stored in liquid nitrogen until the next day. All experiments and in vivo implantation...
10. Plasmid DNA Precipitation: Add 0.63 ml of isopropanol to the eluate. Mix and place on ice for 10 min. Centrifuge the mixture at top speed in a microcentrifuge at room temperature for 20 min. Carefully discard super- natant. Wash the plasmid DNA pellet with 1 ml of ice cold 70% ...
(Fisher Chemical). After rinsing with Milli-Q water, they were sonicated for 30 min, submerged in 1:1 isopropanol/Milli-Q water solution (isopropanol from Fisher Chemical). After thorough rinsing with deionized water, the cover glasses were incubated overnight submerged in a 1:1 sulfuric ...
(Agilent Technologies) was used for lipid analysis. The solvents used were 40% acetonitrile in 60% water with 10 mM ammonium formate (Mobile phase A) and 10% acetonitrile in 90% isopropanol with 10 mM ammonium formate (Mobile Phase B). The peak areas of the lipids were integrated ...