cell physiology, and systems biology can become a powerful tool that maximizes the output of a given cell-free system. Similar approaches have been used for transcriptional rewiring with the aim of increasing the production of proteins and metabolites in vivo33,34. However, in order to refine ho...
Finally, we designed five IL32γ-sensing riboswitches and characterized their ability to repress mRFP1 expression (OFF switches). We directly added 780 nM of purified IL32γ to the cell-free expression system (co-addition). Using our no-aptamer controls, we found no significant non-specific...
In the analysis of high-throughput data from complex samples, cell composition is an important factor that needs to be accounted for. Except for a limited number of tissues with known pure cell type profiles, a majority of genomics and epigenetics data relies on the “reference-free deconvolution...
By means of this work, a promising basis for the application of the hydantoinase process in cell-free reaction systems was accomplished. After improvement of the expression conditions, a hydantoinase and carbamoylase was successfully purified with two different methods. Furthermore, an immobilization ...
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non-neural cells by means of environmental changes, we were not able to increase significantly the percentage of neuronal (GABA-ergic and catecholaminergic) over glial cells under several different cell culture testing conditions. Supplementing serum-free medium with several growth factors (SHH, bFGF,...
( p1304mp , p3566 , p21493 ; nucleic acid stains—section 8.1 ), these nucleic acid stains have been used to discriminate brdu-labeled cells from nonproliferating cells by flow cytometry and with an imaging system for au...
Things like nested tables, merged cells, and conditional formatting can make it difficult for the system to accurately interpret and display the data. Complex formulas and functions can also contribute to formatting issues, especially if they aren't properly structured or if there are circular refere...
The self-replenishing rGPS–MCG cycle designed here enables the production of the key metabolites, such as acetyl-CoA, pyruvate and malate, which can be further converted to almost all chemicals in the biosphere. The cell-free CO2fixation system might become practical if these enzymes can be fur...
S6h). Moreover, in vitro transcribed LNC-SNO49AB could bind to purified Flag-ADAR1 protein in a cell-free system (Fig. 4e). Immunofluorescence assays also showed that ADAR1 locates in the nucleolus, and LNC-SNO49AB and ADAR1 predominantly colocalized in the nucleolus (Fig. 4f and ...