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README License Single cell workflow Identification of cell- and UMI barcodes from single-cell sequencing. Introduction This workflow extracts cell barcodes and UMIs from10x-generated single cell libraries. It was initially created as a Nextflow port ofSockeye. ...
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When the super glue was hardened, dental cement was used to fix a metal bar to the contralateral side of the window. After surgery, the animal received a dose of buprenorphine (0.05 mg/kg) and was allowed to wake up from anesthesia in an incubation box set to 25 °C. The ...
Mine came in darn near perfect condition! The ONLY thing I see wrong is the tiny little chip on the one side. You’ll see in my14th picture. It’s so small it does not bother me one bit. Everything else is in great condition. The camera works perfectly. There are no scratches on...
We added 8 μl 2.5% paraformaldehyde (EMS, 15714-S) to the remaining 2-μl pellet (containing the nucleus), vortexed to resuspend, incubated at room temperature for 10 min to fix nuclei, then added 10 μl 0.25 M glycine supplemented with 0.2 μl magnetic beads (Invitrogen...
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If this plot plateaus out, it may indicate that PCR duplicates represent a high proportion of the reads at higher sequencing depth. Sequencing saturation: calculated as 1 - (number of unique UMIs across all cells / number of reads across all cells). Values near 0 indicate that very few ...
940nm infrared LED system, the camera operates in near-total darkness, providing an unmatched night vision experience. **Reliable and Versatile Performance** This trail camera is designed to withstand the elements, boasting an IP65 waterproof and dust-proof rating, making it suitable for use in ...