For single-nucleus data, the shoot tissues of dark-grown seedlings were frozen in liquid nitrogen, added to nuclear extraction buffer, fragmented with a gentleMACS Octo dissociator (Miltenyi Biotec, 130-095-937), and finally filtered and suspended in 300 μl of 10x wash buffer for nuclear is...
During the preparation of the cell lysate, the concentration of proteins is decreased by a 20–30 factor, and genetic information (i.e., DNA and RNA) is removed from the lysate [75]. For basicE. coliTX-TL reactions, common components are as follows: the cell extract; the buffer system...
Western blotting Cells cultured in 6-well plate dishes were lysed for 30 min in 0.3 ml of 1% Triton X-100 lysis buffer (20 mM Tris-HCL [pH 7.5], 150 mM NaCl, and 1% Triton X- 100) containing 5 μg ml-1 lupeptin, 50 mM phenyl- methylsulfonyl fluoride, and 7.2 trypsin inhibitor...
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Buffer Overflow in C# Build an entire solution programmatically Build C# Application to single EXE file or package Build string.Format parameters with a loop Building an async SetTimeout function button array in c# Button click open Form 2 and close Form 1 Button Events not working Button is Di...
Genomic DNA extraction Genomic DNA of human lung specimens were extracted by using QIAamp DNA Mini kit (QIAGEN, Germantown, MD, USA) according to manufacturer’s protocol. Immunoprecipitation Cells were harvested in protein lysis buffer containing protease inhibitor cocktail (Merck Millipore, Bedford, ...
After the measurement, the liquid flow was switched back to the PBS buffer until a stable baseline was obtained, to wash out bound analyte and prepare the crystal for further use. After the analysis, the quartz was incubated into a culture medium to verify the cellular growth. ...
Western blotting Cells cultured in 6-well plate dishes were lysed for 30 min in 0.3 ml of 1% Triton X-100 lysis buffer (20 mM Tris-HCL [pH 7.5], 150 mM NaCl, and 1% Triton X- 100) containing 5 μg ml-1 lupeptin, 50 mM phenyl- methylsulfonyl fluoride, and 7.2 trypsin inhibitor...
On the day of extraction, cells were also singly treated for 2 h before the extraction to estimate the CUR levels attached or absorbed to the cell surface. The CUR levels in the media and cells were measured using a previous HPLC method with slight modification as described below [16]. ...
However, such a modification of T acts only on the cells which have not yet been received, and not on those already written to the buffer memory of the spacer, given that the theoretical emission times calculated previously are no longer modifiable without knowing the connections concerned; yet...