Although plant cells differ greatly they all have similar eukaryotic organisation.It can be seen in this diagram of a plant cell that the cells are made up of many different parts; these are known as organelles. These are described in more detail below. Parts shown in the Plant cell diagram...
After treatment, FITC-labeled dextran was added to the top of the cell monolayer for 20 min, and then FITC-dextran penetrated from the GEC monolayer to the bottom of the wells was measured by relative fluorescence excitation at 485 nm and emission at 530 nm using a fluorescence plate...
1g). We labeled human (HEK 293T) cells and mouse (expanded iEPs) cells with two different versions of the CellTag-multi library, combined nuclei isolated from both populations in a 1:1 ratio and profiled them using our modified scATAC-seq method. Plotting CellTag reads per cell, we ...
Figure 1. Diagram of uterine leukocyte isolation and purification for in vitro assays. (A) A cell suspension prepared from the mesometrial region (e.g., dissected from pregnant uterus at gd 8.5) is centrifuged over a 30, 40, and 70% Percoll step gradient. The buffy coat on top of the...
trajectory inference withn = 10 random root cells.p-values were computed using a Fisher exact test and adjusted with Benjamini Hochberg correction.fThe venn diagram illustrates the overlap of DE genes across feature selection strategies.gBarplots indicate mean ± standard deviation of the ...
Endocrine cellsare scattered throughout thegastrointestinal mucosafrom the stomach to the colon and constitute one of the largestendocrine systemsin the body.Enteroendocrine cellslabeled bychromogranin Acomprise about 1% of all epithelial cells in thegastrointestinal tractand are surrounded by mucus, chief...
andRemdesivirtreated group. The single-cell dataset used as the reference is from Baron et al.35(GEO accession number:GSE84133) which has 14 labeled cell types in pancreas tissue. Instead of using all the cells in the dataset, we only selected endocrine cells: alpha cell, beta-cell, delta...
In conjunction with propidium iodide (P1304MP, P3566, P21493; Nucleic Acid Stains—Section 8.1), these nucleic acid stains have been used to discriminate BrdU-labeled cells from nonproliferating cells by flow cytometry and wit...
()function in the R package umap. We labeled the cells with their cell types. We reported the miLISI (proposed by Korsunsky et al.44) to quantify the degree of mixing between the synthetic cells and real cells (see the subsection “Evaluation metrics”). The miLISI value (ranging from 1...
3b), thanks to the ultra-brightness of Rdots and optical integration of all Rdots labeled to single cells. Figure 3c shows the corresponding SRS image at 2218 cm−1, and the membrane-bound SRS signal confirms the subcellular localization of CD44 receptors and validates that Rdots ...