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We first clustered RPE1-FUCCI cells into eight groups based on cell cycle stages and calculated the average spliced and unspliced expression of cell-cycle-associated genes, which also have synthesis and degradation rates in scEU-seq (Extended Data Fig. 5a). We applied cellDancer to predict ...
or by elimination of some factors inherited by the cells from previous developmental stages. Here we discuss a particular temporal regulation of the first embryonic M-phase ofXenopusand mouse embryos. These two examples help to understand better the general regulation of M-phase of the cell cycle...
were processed using Cytobank (http://www.cytobank.org/). Additional gating on the DNA channels (191Ir and193Ir) was used to remove remained doublets, debris, and contaminating particulates. Manual gating was performed on IdU, cyclin B1, p-HH3, and p-RB to identify cell-cycle stages32. ...
(Arai et al., 2011). Results from Saade's work also showed that even if the length of theG1 phasedid not change between the developmental stages analyzed, the proportion occupied by the G1 phase was bigger at HH stage 24 and represented 52% of thewhole cellcycle (Saade et al., 2013...
Active extracellular signal-related kinases (ERKs) translocate to the nucleus, where they phosphorylate and stabilize several transcription factors involved in the early stages of the G1-S cell cycle transition. The activation of MET can also induce the translocation of STAT dimers and NF-κb to ...
A second proposal for the importance of cell cycle-regulated transcription centers on building a required structure only once per cell cycle (Fig.2b) (reviewed in [32,33]). For example, proteins required for DNA replication are loaded onto DNA in different stages. The components of the replica...
(refs.22,23). Sorted CD34+TP53-multi-hit cells exhibited potential for erythroid differentiation in vivo and in vitro, supporting that this occurs downstream of the LSC population (Extended Data Fig.5a–c).TP53multihit LSCs showed enrichment of cell cycle, inflammatory, signaling pathways and ...
Using the Fly-FUCCI system [30], we found that 23.8% of polyploid NBs presented nuclei at different cell-cycle stages (Figure 4A). Further, we also observed polyploid NBs in which certain nuclei of a given cell entered and exited mitosis although other nuclei of the same cell remained in ...
CellRank fate probabilities and Palantir pseudotime21are used to define three stages of the dedifferentiation trajectory (top,Methods). Dedifferentiation stages are characterized by expression ofBpifb1(goblet),Krt5(early basal) andTrp63(late basal); stage 1 corresponds to goblet, stage 2 to inte...