FxCycle stains flow cytometry data Figure 5. Multiparametric cell cycle and immunophenotypic analysis. TF-1 erythroblast cells were alcohol-fixed overnight, washed, and then suspended in 0.1% Triton X-100/PBS/1% BSA before staining with anti–histone H3[pS10] ...
Cell cycle arrest is a stopping point in the cell cycle, where it is no longer involved in the processes surrounding duplication and division. 细胞周期阻滞是指细胞周期停留在G1期,而无法有效的完成整个细胞周期。 G1-phase arrest of cell cycle progression provides an opportunity for cells to either...
2014. Cell Cycle Analysis by Flow Cytometry. Hoboken, NJ: WileyDarzynkiewicz Z, Zhao H. 2014. Cell cycle analysis by flow cytometry.Z. Darzynkiewicz, H. Zhao, Cell Cycle Analysis by Flow Cytometry, eLS, John Wiley & Sons, Ltd., 2001...
proteinstothecellcycleposition.Thefourthprocedurereliesonthedetectionof5'-bromo-2'- deoxyuridine(BrdU)incorporationtolabeltheDNA-replicatingcells. KeyWords:Flowcytometry;cellularDNAcontent;cyclins;BrdUincorporation;mitosis. 1.Introduction Avarietyoffloworcytometricmethodstoanalyzethecellcycleprogres- ...
Identification of Cell Cycle Models by Flow Cytometry - ScienceDirectThe autonomous oscillations in yeast continuous cultures are investigated analytically and related to the behaviour of the single cell by means of a suitable modified version of Monod's classical chemostat model. Two main cell phases...
Flow cytometry, the methodology that provides a way to measure physical and chemical attributes of individual cells rapidly and with high accuracy, is wide... Gray,W J. 被引量: 0发表: 1979年 Analysis of cell cycle by flow cytometry. Described are four widely used procedures to analyze the ...
上海玉博生物科技有限公司 主要致力于“Nuclear-ID™ Green Cell Cycle Kit for flow cytometry ENZ-51014-100”的生产销售。多年的“Nuclear-ID™ Green Cell Cycle Kit for flow cytometry ENZ-51014-100”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,
B-cell activationFlow cytometryAntibody-secreting cellTo date, the trout B-cell is poorly defined, as many essential molecular markers are not yet ... P Zwollo,A Haines,P Rosato,... - 《Developmental & Comparative Immunology》 被引量: 81发表: 2008年 The effect of anti-CD40 ligand antibody...
Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4',6'-diamidino-2-phenylindole (DAPI) and deconvolution of the cellular DNA...
“If you are doing cell cycle analysis on a large number of cells, or on a rare cell subset, then traditional flow cytometry is better,” she says. “But if you want to multiplex cell cycle analysis with other immunostaining within the cells, then an imaging cytometer is definitely neede...