Remember, mitosis is the process of cell division, but it’s just a portion of the full cell cycle. Figure 5 shows approximately how long a cell spends in each stage of the cell cycle: Figure 5. The cell cycle consists of interphase and the mitotic phase. During interphase, the cell gr...
Cell cycle arrest/release experiments of MCF7/SKBR3 breast cancer and MCF10 non-tumorigenic cells were used as a surrogate to support the production of proteins relevant to aberrant cell proliferation. Information downloaded from the scientific public domain was queried with bioinformatics tools to ...
We performed PCA using all APs of the three stages (total N=73 cells) to objectively evaluate the temporal changes in global gene expression in APs (Fig. 2c; Supplementary Fig. 8). In the scatter diagram of PC1/PC2 scores, PC1 clearly revealed a large difference between the E11 and ...
Figure 15.4.4 Schematic diagram showing the sequence of events in the strand break induction by photolysis (SBIP) technique. A) Proliferating cells that have incorporated BrdU (*) into newly synthesized DNA are B) expo...
Venn diagram analysis showed more than 80% of co-expressed genes were shared between TM-1 and Jin668 in all major cell-type clusters, including epidermis, cortex, and primary vascular tissue (Fig. 3b), except for phloem sieve elements, which showed the highest proportion of genes specific to...
Genome-wide studies of cell cycle-regulated transcripts in budding yeast indicate that more than 800 genes are periodically expressed (Spellman et al., 1998). More than 300 of those, including the G1 cyclins, CLN1 and CLN2; the S phase cyclins, CLB5 and CLB6; and many genes involved ...
Revealing molecular trajectories of proliferation and cell cycle arrest Recent advances in spatial single-cell profiling technologies8,9,10,11,88,89,90,91,92have enabled the simultaneous measurement of transcriptomic or proteomic signatures of cells, while also retaining additional imaging or array-derive...
In order to integrate the findings, we turned to chromHMM, a platform that can annotate different states based on various epigenetic and related characteristics [36]. T1- and T2-LADs were significantly enriched for the chromHMM heterochromatin state [36] in all cell types, except for T2-LADs ...
the cells were synchronized by a 24-h light/dark cycle, but the peaks of the mRNA levels were not consistent with the timing of chloroplast division. In addition, both of the FtsZ proteins localize at the chloroplast division site throughout the cell/chloroplast division cycle. Thus, it is ...
The last decade has revealed new roles for Cullin-RING ubiquitin ligases (CRLs) in a myriad of cellular processes, including cell cycle progression. In addition to CRL1, also named SCF (SKP1-Cullin 1-F box protein), which has been known for decades as an