and sectioned, followed by staining with H&E (Beyotime, Shanghai, China), trichrome (Masson) stain kit (Baso Diagnostics Inc., Zhuhai, China), or ORO staining (Beyotime, Shanghai, China). For semi-quantitative analysis of severity of liver fibrosis, five randomly selected fields per ...
Flow cytometric analysis All the transfected CRC cells were harvested after incubation for 48 h. Then the cells were fixed in pre-chilled 70% ethanol for 16 h at 4 °C and Cell Cycle Analysis Kit (Beyotime, China) was used to stain cells for 30 min with propidium iodide accordin...
Lipid peroxidation was evaluated using the malondialdehyde (MDA) assay kit (Beyotime, China) following the manufacturer's instructions. 2.9. Cell proliferation assay To determine cellular toxicity, the CellTiter 96 Aqueous One Solution Kit (MTS Kit, Promega) was used according to the manufacturer's ...
The protein concentration in the supernatant was determined by bicinchoninic acid (BCA) method using BCA Protein Assay Kit (Beyotime biotechnology, Shanghai, China). Protein digestion and TMT labeling One hundred microgram of proteins were resuspended with tetraethylammonium bromide (Haihang Industry, ...
Cell-cycle analysis To evaluate the potential enrichment of mitotic cells in the primary LCs scRNA-seq datasets, we first scored each cell based on aggregated expression of curated S phase and G2/M phase cell cycle genes. During the scaling process of the normalized expression matrices, we “...
-2B cells. Through RT-qPCR and Western Blot analysis, siRNA-FTL#3 showed reliable knockdown ability (Fig.9cand Supplementary Fig.12). Considering the relationship between cell vitality and cellular senescence73, we used Cell Counting Kit-8 (CCK-8) to detect the effect of FTL on cell ...
one-way analysis of variance CK-18: Cytokeratin-18 DMEM: Dulbecco's modified Eagle's medium EMT: epithelial-to mesenchymal transition FBS: fetal bovine serum HCS: high content screening HGF: hepatocyte growth factor HK-2 cells: Human proximal tubular epithelial cells LSD: the least ...
We first performed apoptosis-related protein detection, cell cycle analysis using Propidium Iodide (PI) staining, and Annexin-V/PI apoptosis detection by flow cytometry for the A375 cells. These results all showed that treatment with HC067047 or Baicalin could not induce apoptosis or change cell ...
The intracellular Ca2+ in yeast cells were labeled in situ with specific fluorescent probe Fluo-3-AM (Beyotime Biotechnology Institute, Haimen, China). Fluo-3-AM was added to yeast suspension at a final concentration of 150 μM. Then, the suspension was incubated at 37 °C for 1 h. Confo...
The mice were sacrificed via cervical dislocation on day 1, 8 and 15 for HE staining analysis. Briefly, the mouse dorsal skin was fixed in 4% paraformaldehyde (Beyotime) at 4 °C, subsequently embedded in paraffin blocks, and prepared as 5-µm-thick sections based on the longitudinal ...