流式(FACS)细胞周期分析 细胞周期是细胞复制过程中发生的一系列事件,细胞周期的紊乱可能导致肿瘤的形成。因此,细胞周期的监测可以为疾病诊断和治疗预后提供诸多信息。 G0是一个休止阶段,在此阶段细胞已离开周期并停止分裂。细胞周期可分为以下四个阶段: 流式细胞术检测方法的原理 流式细胞术检测方法受到广泛应用,其原...
Cell cycle analysis of MSCs by FACS and the percentages of cells at different cell cycle phases in different concentrations of bFGF.JunJie, WangYuLiang, LiuYuanChao, SunWei, GeYongYong, WangPaul, W. DyceRong, HouWei, Shen
In addition, other markers of cell cycle progression can be multiplexed with the FITC BrdU flow kit, thereby providing additional multiparametric analysis of the cell cycle using flow cytometry. BD FACSVerse systems include the cytometer, BD FACSuite software for acquisition and analysis, and BD ...
Cell cycle analysis Cell cycle analysis was performed on FACS-sorted cells or on cells recovered from 96-well flat-bottom microplates (BD) after culture on flat hydrogels. Cells were fixed and permeabilized using Cytofix/Cytoperm kit (BD Biosciences). Cells were labeled with Ki67 FITC (BD Biosc...
Cell cycle analysis was performed as previously described[15,16]. Confluent cell cultures were semisynchronized by contact inhibition and serum starvation for 48 h. Subsequently, cells were incubated in serum and removed at specific times, washed in Dulbecco's phosphate-buffered saline (DPBS), tryps...
Cell cycle analysis by 5-ethynyl-2’-deoxyuridine (EdU) labeling revealed a high percentage of S-phase re-entry by overexpression of the entire pool of candidate genes. Subtractive pools identified known cell cycle protein E2f2 as sufficient for S-phase re-entry in cultured adult mouse cardiomyoc...
*Quantitativeanalysisofthenumberofmoleculesofaparticularantigenfoundonthesurfaceofacell(MESF) *Celldivisionstudiesusingantibodiestobromodeoxyuridine(BrdU) *DNAandcellcycleanalysis,DNAIndexforaneuploidy *Mutantisolation *Plantcellstudies *Virusinfectedcells
G1 phase regulation quiescence cell cycle reporter quantitative single cell imaging automated image analysis Introduction Cell fate decisions, such as reprogramming, differentiation, and cell cycle exit, are tightly linked to cell cycle kinetics. Moreover, the responsiveness of cells to internal and exte...
Mouse embryonic cell cycle A dataset of mouse embryonic stem cells undergoing different stages of the cell cycle was accessed from Buettner et al. [36] with the accession code E-MTAB-2805. FACS cell cycle labels from Hoesct flow sorting were used to annotate cells along three phases: G1, ...
Cell cycle analysis after ZNF471 overexpression showed a significant decrease in S-phase cells with a concurrent increase in G2/M phase cells as opposed to control cells (P<0.05; Fig. 3h). Ectopic expression of ZNF471 in SiHa cells showed significantly higher apoptotic cells compared with vecto...