1. 1. 本外观设计产品的名称:细胞培养换液装置;2. The exterior design of the product name: cell culture medium was changed equipment; 2. 本外观设计产品的用途:本外观设计产品用于细胞培养;3. The product design Uses: This product design for cell culture; 3. 本外观设计产品的设计要点:产品的形状;...
Hayflick expected that normal cells would change and display cancer‐like properties, but normal cells did not grow any longer. Hayflick thought that he made a mistake in culture medium composition, glassware cleaning or other technical procedures. A few years later (in 1961), when working with ...
To keep cells in optimal culture conditions and actively growing, it is necessary to renew the growth medium and to subculture them at regular intervals. Change of culture medium can take place several times in the log phase dependent on the cell type. The best time to subculture cells...
The invention provides a cell culture medium additive used for highly producing monoclonal antibodies. The cell culture medium additive is characterized by including following components: 292-1461 mg/L of L-glutamine, 6.5-9.5 mg/L of adenine, 6.5-9.5 mg/L of guanine, 6.5-9.5 mg/L of uracil...
Cells are grown at 37 °C in 7.5% (v/v) CO2 in air for 3 days, at which point medium is aspirated and replaced with new medium. At culture day 7 (C7), medium is aspirated and a solution containing 2 ml 0.1% trypsin is added to the 100 mm dishes or 1 ml is added to the ...
网络释义 1. 细胞培养基 Biowest公司简介 -... ... Trypan Blue 台盼蓝Cell Culture Medium细胞培养基Salts Solutions,Salts/Buffers 盐溶液 ... www.bioconsumable.com|基于23个网页 2. 细胞培养液 (2)细胞培养液(cell culture medium) (3) 直径 9 公分大之细胞培养皿 ( 9-cm culture dish)内皮细胞培...
Culture conditions vary widely for each cell type, but the artificial environment in which the cells are cultured invariably consists of a suitable vessel containing the following: A substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals) ...
Cell culture medium was changed every 3 days. Animal Female C57BL/6 J mice (18 ± 5 g) were obtained from Central Laboratory Animal Inc. (Seoul, Korea). Animals were kept in a 12-h light–dark cycle, with controlled temperature (22–24 °C) and humidity (50–60%) and ...
Cell culture medium was changed every 3 to 4 days and cells were subcultured using trypsin-EDTA (PAA Laboratories) before reaching confluence. To stimulate elastogenesis, fibroblasts were seeded in appropriate culture dishes with a density of 20.000 cells/cm2 and treated with 10 ng/mL TGF-β...
aThe culture medium including the bone marrow was filtered by a 40 lm cell strainer (BDBioscience) and centrifuged at 400 9 g for 5 minutes 培养基包括骨髓由40 lm细胞过滤器BDBioscience (过滤) 并且被分离了在400 9 g在5分钟[translate]