This protocol describes a bio-orthogonal method for dynamically altering the adhesiveness or stiffness of the synthetic extracellular matrix during three-dimensional culture in a spatiotemporal manner to induce phenotypic changes and to produce functional tissues. ...
Cite this protocol Shen, J., Lee, V.H.L. (2002). Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract. In: Wise, C. (eds) Epithelial Cell Culture Protocols. Methods In Molecular Medicine™, vol 188. Humana Press. https://doi.org/10.1385/1-59259-185-X:...
Cell culture models for drug transport studies. In: Wang B, Siahaan T, Soltero R, editors. Drug Delivery: Principles and Applications . Hoboken, NJ, USA: John Wiley & Sons; 2005. pp. 103–124.Karunaratne DN, Silverstein PS, Vasadani V, Young AM, Rytting E, Yops B, Audus KL. ...
It is also used in drug screening and development, and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins). The major advantage of using cell culture for any of these applications is the consistency and reproducibility of results that can be obtained fr...
It is also used in drug screening and development, and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins). The major advantage of using cell culture for any of these applications is the consistency and reproducibility of results that can be obtained ...
and mutagenesis and carcinogenesis. It is also used in drug screening and development, and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins). The major advantage of using cell culture for any of these applications is the consistency and reproducibility of...
Indeed, both cell culture and transport experiments across epithelial cell monolayers have been automated, and these models are now used as a screening tool in drug discovery programs in many drug companies for the prediction of intestinal drug permeability. 展开 ...
The invention also discloses a cell culture prepared by the preparation method, and an application of the cell culture in the drug screening. The cell culture can be applied to drug screening, and can be used to decrease the drug effect difference between the in vitro and vivo; moreover, ...
Cell culture model for drug bioavailability 专利名称：Cell culture model for drug bioavailability 发明人：Paul B. Watkins,Phyllissa Schmiedlin-Ren 申请号：US08/779596 申请日：19970107 公开号：US05856189A 公开日：19990105 专利内容由知识产权出版社提供 摘要：A model utilizing cells for assessing oral ...
Drug exposure time is a crucial difference between the cell culture experiments and the in vivo situation after topical administration. After topical ocular administration, the drug concentration in the tear fluid drops rapidly within a few minutes, but the drug may be applied continuously using multi...