Here we report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support cancer cell proliferation in environments ranging from standard culture media to tumours. We applied this platform to identify the transporters of amino acids ...
Prior to transfection, 1.3–1.5 × 106 ABM 293 T cells were transplanted into 10 cm cell culture dish in advance and cultured. 70–80% fusion rate was achieved at the time of transfection. Gene Primer sequence(5′-3′) DCXNLS1 del CS31818-FP: GCATCTAGAAATATGAGAGGGTCATCAACG...
Prior to transfection, 1.3–1.5 × 106 ABM 293 T cells were transplanted into 10 cm cell culture dish in advance and cultured. 70–80% fusion rate was achieved at the time of transfection. Gene Primer sequence(5′-3′) DCXNLS1 del CS31818-FP: GCATCTAGAAATATGAGAGGGTCATCAACG...
HEK 293T cells grown in a 15-mm glass-bottomed cell culture dish (Nest Biotechnology, China) were transfected with 1 μg of plasmid expressing hamster LDLR-Flag or LDLRAD3-Flag (control). At 24 h post-transfection cells were incubated with rGETV-mCherry (MOI of 200) at 4°C or ...
Cells were centrifuged for 7 minutes at 250g, the supernatant aspirated, and resuspended in culture medium. Cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low density lipoprotein (Stemcell ...
To investigate the mechanisms of RCC recruitment of mast cells to enhance angiogenesis, we first used transwell co-culture migration assays (Figure 3a). The results revealed that RCC OSRC-2 cells could recruit more HMC-1 cells than normal kidney HK5 cells and RCC 769-P cells (Figure 3b). ...
Finally, though we have identified extracellular signals that specify human mesoderm cell fate in vitro, to accompany the jump of complexity from 2D culture dish to 3D embryo we must in turn map the niche cells that produce these signals during embryogenesis and where they are located, thus ...
i Cartoon depiction of the average order of events for a cell that reverses. Source data are provided as a Source Data file. Full size image Moreover, as the stimulated original rear began to increase local Cdc42 activity, the two cell edges pulled in competing directions, causing the ...
In 6-cm culture dish, the HepG2 cells were treated with siNC or siSTX18 for 45 h. Then cells were washed three times with PBS and incubated in phenol red-free, serum-free F12 medium (Gibco) containing 1% fatty acid-free BSA in the presence or absence of 500 nM isoproterenol (...
Cell culture and reagents Jurkat cells and J-Lat 11.1 cells (Jurkat cells containing integrated full-length HIV-1 genome mutated inenvgene and GFP replacingnef)39were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 7% FBS and 100 μg/ml penicillin-streptomycin at 37 °C ...