AWestern blot was performed to determine the protein levels of SIRT1 in NRCMs transfected with short interference SIRT1 (si-SIRT1) or Scramble (si-Scr).BRepresentative immunofluorescence (red for cTnT, blue for DAPI) and quantitative analysis of myocyte surface area in NRCMs transfected with s...
j Quantification of the fibrotic area (n = 10 per group). k Representative heart sections stained with H&E (top) and WGA (bottom) to detect the presence of hypertrophy in cardiomyocytes. l Quantitation of relative cardiomyocyte cross-sectional area (n = 10 per group). m, n WB ...
Average size of the nucleolus (cross-sectional area in μm2) and percentage of granular component (GC). A)Two-day experiment.B)Four-day experiment. Statistically significant differences in nucleolar size (p < 0.05), compared to the control, are indicated with a ‘§’ symbol when compar...
Conversely, overexpression of SND2 in Eucalyptus stems led to increased fibre cross-sectional cell area. Conclusions This study supports a function for SND2 in the regulation of cellulose and hemicellulose biosynthetic genes in addition of those involved in lignin polymerization and signalling. SND2 ...
On the other hand, a single vertical structure led to high electrode impedance because of the decrease in the cross-sectional area [97]. Overall, the application of 3D nanodevice can achieve a remarkable cell–electrode coupling efficiency for intracellular recordings. To improve the performance of...
Finally, the spatial resolution was evaluated with size standard particles, showing a good correlation between the particle diameter squared and the calculated cross-sectional area of the particle from 2.0 μm to 10.1 μm (Figure S3H). These results demonstrate the FDM microscope is capable of ...
Sections were stained with 50 μg/ml WGA (Sigma, USA) to measure cardiomyocyte cross-sectional area. After transfection and hydrogen peroxide stimulation, H9C2 cells were fixed with 4% paraformaldehyde and cells were incubated with 50 μg/ml WGA for 30 min at 37 °C. Images were observed ...
Reasonable geometric structure of the cross-sectional area of the TTEM cell would thereupon be resolved. Numerical results of the local higher order modes along the length of the cell are presented in this paper. 展开 关键词: EMC measurement TEM cell finit element method higher order mode ...
L Quantitative analysis of the myocyte cross-sectional area (μm2). M Western blot analysis of key molecules of Bmp10-mediated Smad-dependent signaling. N Quantitative analysis of the expression of Type I collagen/GAPDH, Type III collagen/GAPDH, and p-Smad1/5/8 (at Ser463/Ser465/Ser467)...
3d (y = 0.95x − 13.55), where x is muscle fibre cross-sectional area. Combining equations for fibre cross-sectional area and myofibril cross-sectional area allows calculation of myofibril cross-sectional area from fibre diameter (D): $$0.95\pi \left( {\frac{D}{2}} \right...