Primary (new) cultures are more representative of responses of their plant species but produce nonuniform suspension due to clumping and clumping presents unavoidable sampling difficulties. Evaluations were made of the response of plant cell suspension in primary cultures subjected to metal stress in ...
Besides the difficulty in getting the serum-free conditions just right, other adverse effects of going serum-free include slower cell growth and cell clumping when passaging. Continued optimization of culture conditions can help with the reduced rate of growth, and gentle repeated pipetting of cell...
(pre-chilled to −20 °C) for 10 min on ice in the dark. Methanol was added dropwise with a gentle vortex to avoid cell clumping. Fixed cells were centrifuged at 900 g for 3 min at 4 °C. Cell pellet was washed (not re-suspended) with ice-cold PBS (with 0.4 U/μ...
Swelling and clumping: Infected cells greatly expand and clump together in “grape-like” clusters before detachment. It is characteristic of adenoviruses. 5. Foamy degeneration: It is due to the production of many large cytoplasmic vacuoles. For example, certain retroviruses, paramyxoviruses, and fl...
Distinct, possible targets within the realm of the cell wall arechitinases, enzymes that are essential for cell-wall plasticity during growth and proliferation (Cabib, 1987). Disruption of the genes encoding for chitinase inSaccharomyces cerevisiaeresults in clumping of the cells and the lack of se...
Upon addition of the harvesting solution, the cells of both donors started to detach and detached cells appeared in the intensity images as bright regions, with considerably more clumping behaviour for donor 6. Towards the end of the harvesting assay, the majority of the cells were detached from...
DNase was added to prevent cell clumping. Cells were then centrifuged to remove PEG, washed twice in RPMI 1640, and resuspended in complete medium (RPMI 1640 + 10% fetal calf serum + 2 mM glutamine, penicillin, and streptomycin). For the two steps procedure, labeled cells were initially ...
上海玉博生物科技有限公司 主要致力于“RECOVERY CELL CULTURE FREEZING Invitrogen-12648010”的生产销售。多年的“RECOVERY CELL CULTURE FREEZING Invitrogen-12648010”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖
phase (3.3–3.4 C), (e) the exclusion of chromatin bodies and the formation of clusters of large-sized perichromatin granules in late S phase (3.5–3.8 C) and (f) large extensive disruptions and holes in the nuclear membrane and the clumping of incompletely folded chromosomes (3.8–4. C...
(BioGems, Cat: 5000222-5G)). Cells were first seeded in a low binding plate for 4 h at 37 °C, 5% CO2, to promote cell clumping, then clumps were centrifuged, resuspended in pure Matrigel® (Corning, Corning, NY, Cat: 356231) and 25 µL domes were seeded in 48-well ...