Cell clumping occurs when cells aggregate in a culture and begin to block each other from resources. Learn more here.
Cell culture glassware should always be cleaned with phosphate-free detergents and be thoroughly rinsed, as residual levels of cleaning detergents in vessels can be toxic to cells. Over time, spinner flasks can develop a film of surfactants and cell debris. These vessels should be periodically stri...
The present invention relates to cell culture media comprising polymer embedded components. Some components show poor stability in dry powder cell culture media. Others are hygroscopic. Such components can be embedded into polymers to increase stability and reduce clumping.RAYNER-BRANDES Michael Howard...
Cell clumping often occurs during adaptation to SFM. We recommend that you gently triturate the clumps to break them up when passaging cells. Morphology It is not uncommon to see slight changes in cellular morphology during and after adaptation to SFM. As long...
PB180523 Poly-L-Lysine Solution, 10 × 10mL P-CC-001 Room SafeGuard 480mL P-CC-002 Bath SafeGuard 100mL PB180518W Water For Injection (WFI) for Cell Culture 500mL PB180526P Yeast extract,powder 10g PB180527 Anti-cell clumping agent 10mL PB180528 Poloxamer 188 Solution 100mL 推文 關於...
Adding poly-d-lysine to laminin resolves the clumping, though these cell cultures are still less homogeneous than treating surfaces with solely poly-d-lysine or poly-amine. Scalebar is 200 μm. In the following, we describe the maintenance of neuronal forebrain cultures isolated from newborn ...
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Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Place at 37°C to facilitate dispersal. 7. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture ...
DNase was added to prevent cell clumping. Cells were then centrifuged to remove PEG, washed twice in RPMI 1640, and resuspended in complete medium (RPMI 1640 + 10% fetal calf serum + 2 mM glutamine, penicillin, and streptomycin). For the two steps procedure, labeled cells were initially ...
Karcy, "aggregate cell culture"确实是一种培养方法,不是特性描述。稍一google你就看到了。比如: "... model of aggregate cell culture, a culturing technique. in which cells are reaggregated into small three di-. mensional masses. ..." http://www.google.com/url?sa=t&ct=res&cd=16&url=ht...