View chapter Explore book Tumor Immunology and Immunotherapy – Cellular Methods Part B GiuliaCerrato, ...GuidoKroemer, inMethods in Enzymology, 2020 7Bone marrow-derived dendritic cells (BMDC) culture 1. BMDC are isolated postmortem from C57BL/6 using a sterile scalpel. Femur and thetibiaare ...
View chapterExplore book Development of T Cell Immunity Michelle A.Linterman,Carola G.Vinuesa, inProgress in Molecular Biology and Translational Science, 2010 Abstract HelperT cellsare required for the generation of a potent immune response to foreign antigens. Amongst them, T follicular helper (Tf...
Chapter © 2021 Development of a 48-Well Dynamic Suspension Culture System for Pancreatic Differentiation from Human Embryonic Stem Cells Article 02 December 2021 Background Human-induced pluripotent stem cells (iPSCs) possess the potential to revolutionize the field of regenerative medicine, offeri...
2.1.912 Part 3 Section 19.785, text:continue-list 2.1.913 Part 3 Section 19.786, text:continue-numbering 2.1.914 Part 3 Section 19.788, text:count-empty-lines 2.1.915 Part 3 Section 19.789, text:count-in-text-boxes 2.1.916 Part 3 Section 19.790, text:current-selected 2.1.917...
a normal distribution, the use of a Student’s t-test for large numbers may be not appropriate. Hence, we also describe the difference in their distributions. For both astrocyte and immune cell classes, more than half of the cells of each class exhibited a lower UMI count than the lowest...
Chapter Google Scholar Winding P, Berchtold MW. The chicken B cell line DT40: a novel tool for gene disruption experiments. J Immunol Methods. 2001;249:1–16. Article CAS PubMed Google Scholar Shinohara H, Yasuda T, Aiba Y, Sanjo H, Hamadate M, Watarai H, et al. PKCβ regulates...
In this chapter, we focus on intestinal epithelium to highlight how cell-cell junction proteins, especially AJ proteins, interact together in normal and cancerous contexts to regulate major cellular processes such as cell differentiation and migration. For instance, imbalance in the signaling ...
Chapter Google Scholar Isensee F, Jaeger PF, Kohl SAA, Petersen J, Maier-Hein KH. nnU-Net: a self-configuring method for deep learning-based biomedical image segmentation. Nat Methods. 2021;18:203–11. Article CAS Google Scholar He K, Gkioxari G, Dollár P, Girshick R. Mask R-CNN....
Unfortunately the therapeutic potential of cord blood is also being exploited commercially and not always with the best intentions (see also Chapter 9, Stem Cell Tourism). Various companies, especially in the United States, now offer to collect and freeze cord blood for future private use by the...
As already mentioned in the chapter called Cells of bone, osteocytes are the most common cell type in bones and represent fully matured bone cells that are embedded within the mineralized bone matrix. Osteocytes are distinctive in their stellate-shaped morphology, with numerous dendrites extending ...