...done Generating STAR genome index (may take over 8 core hours for a 3Gb genome)... Failed to make genome index with STAR. This can occasionally be caused by setting the argument `memgb` too low. Error was from running command '/work/b/cellranger-7.1.0/lib/bin/STAR' Command '[...
Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, several CellTracker fluorescent...
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c Working model showing potential roles of PIM1 in hepatic lineage reprogramming. Full size image In the TPCW, 24 downstream TFs that were predicted to be directly regulated by FHH were found to be significantly up-regulated during the early stage of hepatic conversion (Fig. 7a). We counted ...
17-ODYA (Tocris Bioscience, 06-171-0) was dissolved in DMSO and a working stock solution of 4 mM was prepared by 1:6 complexing to BSA (Sigma-Aldrich, A6003). 13C-EdU was synthesized as the previous reported53. AltQ2 (a generous gift from Professor Mikiko Sodeoka) was dissolved in...
in Cell A2: Activity - User writes here hours how much he was working. in Cell A3: Activity - similar as in A3 in Cell A4: Hours - there is sum of hours that user spent on project. so in my Excel I took value A1 to get project name and value from A4 to get hours and it ...
If the workflow fails please run it with the demo data set to ensure the workflow itself is working. This will help us determine if the issue is related to the environment, input parameters or a bug. See how to interpret some common nextflow exit codeshere. ...
After removing labeling medium, cells were fixed and incubated with anti-BrdU-POD working solution for 90 min. Cells were washed 3 times with washing solution and added substrate solution and incubated for 3-10 min at room temperature. The incorporation of BrdU during DNA synthesis in ...
BEIJING, March 20 (Xinhua) -- Chinese researchers have independently developed automobile fuel cell modules with over 5,000 hours durability. The hydrogen fuel cell product, developed by Sunrise Power based in northeast China's Dalian, can be switched on below minus 10 degrees Celsius and reserve...
After injection the cells are again transferred to 1X MBS medium and stored at 16 °C for optical imaging. The cell medium is changed every few hours to prevent bacterial growth. All our experiments complied with all relevant ethical regulations and were approved by the Institutional Animal ...