Abdel-Azim, H., Elshoury, A., Mahadeo, K. M., Parkman, R. & Kapoor, N. Humoral immune reconstitution kinetics after allogeneic hematopoietic stem cell transplantation in children: a maturation block of IgM memory B cells may lead to impaired antibody immune reconstitution. Biol. Blood Marrow...
TruStain FcX block (BioLegend, 422301) was added before mixing and incubating for 10 min on ice. Next, 1 µg of TotalSeqA antibody was diluted in cell staining buffer, and 50 µl of this antibody dilution was added to the blocked cells before incubation on ice for 30 min....
Abe K, Yoshida M, Usui T, Horinouchi S, Beppu T (1991) Highly synchronous culture of fibroblasts from G2 block caused by staurosporine, a potent inhibitor of protein kinases. Exp Cell Res 192:122–127 Article PubMed CAS Google Scholar Schnier JB, Nishi K, Goodrich DW, Bradbury EM (...
Full size image Flagellin is the main building block of the organs responsible for moving almost all flagellated bacteria and the primary ligand of receptors like FLS2 (Schuster and Khan1994). Flagellin perception depends on FLS2 heterodimerization with the LRR-RLK co-receptor BRASSINOSTEROID INSENSITI...
The common gene block sequence used is previously published (Benci et al., 2019). For human cell lines, cells were stably transduced with Cas9 using the lentiviral EFS-Cas9-P2A-Puro expression vector (Addgene #108100) (Tarumoto et al., 2018). sgRNAs targeting human RIPK1 (hRIPK1) or...
These results suggest that U73343 is able to rapidly block both the MTX-induced change in [Ca2+]i and COP formation when added shortly after MTX. When U73343 is added at longer times, a large proportion of the cells challenged by MTX have apparently passed a point-of-no-return and are...
In HeLa-NC cells, γH2AX levels gradually increased 5 h after release from the G1 block, and reached a peak at 8-10 h, i.e. with the majority of cells in G2/M-phase. This cell cycle associated increase of γH2AX levels in the DNA-PKcs-depleted HeLa-H1 cells was much lower than...
For BrdU immunofluorescence staining, samples were pretreated with 1 M HCl for 30 min at 37°C before the application of block solution. The primary and secondary antibodies used in this study can be found in key resource table. Behavioral tests At the age of 8-week, NestinCreERT2::Ogt...
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