TruStain FcX block (BioLegend, 422301) was added before mixing and incubating for 10 min on ice. Next, 1 µg of TotalSeqA antibody was diluted in cell staining buffer, and 50 µl of this antibody dilution was added to the blocked cells before incubation on ice for 30 min....
Rabouw, H. H. et al. Inhibition of the integrated stress response by viral proteins that block p-eIF2–eIF2B association. Nat. Microbiol. 5, 1361–1373 (2020). Article CAS PubMed Google Scholar Cameron, C. M., Barrett, J. W., Mann, M., Lucas, A. & McFadden, G. Myxoma virus...
Flagellin is the main building block of the organs responsible for moving almost all flagellated bacteria and the primary ligand of receptors like FLS2 (Schuster and Khan1994). Flagellin perception depends on FLS2 heterodimerization with the LRR-RLK co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED ...
dissolved CO2partially reacts with water to form H2CO3, which dissociates into HCO3−and H+. The balance between H2CO3and HCO3−levels underlies the most important mechanism of maintaining blood pH; H2CO3and HCO3−are able to neutralize excess bases and acids, respectively...
(H and I) Distribution of inferred cell-specific parameters alpha (H) and beta (I) in Biscuit across cells from each patient. These differences were corrected in normalizing using these alpha and beta parameters. To assign each cluster to a cell type, we compared cluster mean expression to ...
Single cell suspensions were first incubated with FcR Blocking Reagent (Miltenyi Biotec) in staining buffer (2% FBS, 0.1% Na azide, in PBS) for 10 min at 4°C to block binding to Fc receptors. Then the cells were stained with proper antibodies or matched isotype controls in staining buffer...
Cell elongation is mainly limited by the extensibility of the cell wall. Dicotyledonous primary (growing) cell walls contain cellulose, xyloglucan, pectin and proteins, but little is known about how each polymer class contributes to the cell wall mechani
Louis, MO, USA, 45 mg kg-1) 1 h before they were killed. For immunostaining, the cryosections were fixed in cold acetone (4°C) for 10 min and then incubated for 30 min in a 1% bovine serum albumin (BSA)/PBS solution to block non-specific binding. For BrdU staining, the cryo...
[8]. Recent single molecule experiments show that the MRN complex slides along the dsDNA and activates ATM when MRN hits a road block, such as KU at the DNA ends [95]. Prior biochemical studies also suggest that MRN unwinds the DNA ends and activates ATM via the ssDNA region [14]. ...
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