STA-383 Cell Biolabs 的人白蛋白 ELISA 试剂盒是一种酶免疫测定法,用于检测和定量血浆、血清、尿液或其他生物液体样品中的人白蛋白。该试剂盒的检测灵敏度限为 5 ng/mL 人白蛋白。每个试剂盒提供足够的试剂来执行多达 96 次检测,包括标准曲线和未知样品。 丙an酸转氨酶 (ALT) 活性测定试剂盒(比色法) 100...
After the last wash, 50 μl of 1× SDS-PAGE blue loading buffer (New England Biolabs) was added to the bead pellet before loading. Signals were detected with an enhanced chemiluminescence system (GE Healthcare, Chicago, IL) and quantified using a LAS-4000 image analyzer (Fuji Film, ...
Nuclear pellets were resuspended in 500 µl 1.2x NEB2.1 (New England Biolabs (NEB)) with 0.3% SDS, transferred to low-retention tubes (Eppendorf Protein Lo-Bind) and incubated at 37 °C for 30 min in a ther- momixer (950 rpm). The temperature was then adjusted to 60 °C and ...
Nuclear pellets were resuspended in 500 µl 1.2x NEB2.1 (New England Biolabs (NEB)) with 0.3% SDS, transferred to low-retention tubes (Eppendorf Protein Lo-Bind) and incubated at 37 °C for 30 min in a ther- momixer (950 rpm). The temperature was then adjusted to 60 °C and ...
货号:K-CellG5 包装:1 Kit 英文名:Cellulase Assay Kit (CellG5 Method) 品牌:Megazyme Megazyme纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method), 货号:K-CellG5, 用于比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶(纤维素酶)的含量。
The assays were performed by using PAK1 PBD agarose- beads (Cell Biolabs, STA-411) according to the manufacturer's instructions. Cell lysates were obtained from one 100 mm plate from fibroblasts or tumour cells. The lysis buffer used was 50 mM Tris pH 7.5, 200 mM NaCl, 2.5 mM MgCl2, ...
The ROCK activity assay was tested by a 96-Well Rock activity assay kit (STA-416, Cell Biolabs, United States). The lysates of cells were placed in wells that were pre-coated with recombinant MYPT1 protein and detected with the anti-Phospho-MYPT1 (Thr696) antibody (STA-416, Cell Biolabs...
First-strand complementary DNA (cDNA) syntheses were performed at 25°C for 10 minutes, 42°C for 15 minutes, and 70°C for 15 minutes, using random hexamers and ProtoScript II Reverse Transcriptase (New England BioLabs Inc.). In a second-strand cDNA synthesis, the RNA templates were ...
Restriction enzymes were from New England Biolabs (Ipswich, MA, USA). Vectors and inserts were purified using the Wizard PCR & Gel cleanup kit (#A9281, Promega, Madison, WI, USA). Ligations were performed using the DNA Ligation kit 2.1 (# 6022, TaKaRa Bio, Shiga, Japan). All plasmids...
Chemo-activation of mitochondrial ClpP exhibits promising anticancer properties. However, we are currently unaware of any studies using selective and potent ClpP activators in lung squamous cell carcinoma. In this work, we report on such an activator, ZK