A total of 1 × 105cells were plated overnight in a six-well plate containing cell slides. The next day, the cells were fixed with 4% formaldehyde for 20 min at room temperature (RT). Subsequently, the cells were washed three times with PBS and incubated with buffer (2% BSA plu...
aIt shows how DNA tells the cell to built its parts, the words in the DNA map, but we still do not understand what they all do. 它显示怎么脱氧核糖核酸在脱氧核糖核酸地图告诉细胞对建立了它的零件,词,但我们仍然不了解什么他们全都。[translate]...
To understand what this means, consider the following: When a mutation gives a cancer cell a growth advantage, it can make more copies of itself than a normal cell can — and its offspring can outperform their noncancerous counterparts in the competition for resources. Later, a second mutation...
In this review, we concretely describe the characteristics of the SASP and its regulation mechanisms. Importantly, we provide novel perspectives on how the SASP can promote atherosclerosis. The SASP from different types of senescent cells have vital roles in atherosclerosis progression. As a significant...
and its downregulation is associated with decreased reprogramming efficiency. In addition, sialylation levels and patterns are altered during cancer progression, indicating the potential of sialylated molecules as cancer biomarkers. Taken together, the current evidences demonstrate that sialylation is involved ...
The effect of background noise and its removal on the analysis of single-cell expression dataPhilipp Janssen, Zane Kliesmete, Beate Vieth, Xian Adiconis, Sean Simmons, Jamie Marshall, Cristin McCabe, Holger Heyn, Joshua Z. Levin, Wolfgang Enard & Ines Hellmann ...
3d,e). Therefore, F-Cell specification is a post-mitotic event and displays plasticity before the activation of neur expression. The association of the F-Cell with the bristle, and its proximity to the socket and shaft cells (Fig. 1e and Extended Data Fig. 2), suggests that F-Cell ...
BANKSY leverages the fact that a cell’s state can be more fully represented by considering both its own transcriptome and that of its local microenvironment. BANKSY uses a pair of spatial kernels to encode the transcriptomic texture of the microenvironment, one constructed using the weighted mean ...
The testes of sexually mature males of six mammalian species (men, bulls, boars, rats, mice, guinea pigs) have been studied using biochemical as well as light and electron microscopical techniques, in particular immunolocalizations. In these tissues, the peritubular walls represent lamellar encasemen...
Although the function of rab1 is still not fully understood, several lines of evidence describe rab1 and its yeast homolog Ypt1p GTPase as a major component in a pro- tein complex responsible for directing vesicular traffic between the ER and the Golgi apparatus [7-13]. In the past, ...