Cell analysisMass spectrometryMetabolismCell signalingChip-MS platformMicroenvironmentMigrationSubcellular analysisBenefit from the development of microfluidic chip and mass spectrometry, chip-mass spectrometry (Chip-MS) has become an ambient platform for cell analysis. Microfluidic chip has been demonstrated as...
Benefit from the development of microfluidic chip and mass spectrometry, chip-mass spectrometry (Chip-MS) has become an ambient platform for cell analysis. Microfluidic chip has been demonstrated as a powerful tool for cell studies, where identical cells or multiple types of cells were co-cultured...
In this review, we highlight the latest development of multi-channel microfluidic chip-mass spectrometry(chip-MS) in cell analysis and metabolite detection. Following a brief introduction about history and development of multi-channel microchip and MS combination, we will elaborate the key issues of...
It facilitates cell isolation, imaging, and protein preparation for mass spectrometry analysis, including TMT labeling. Deep coverage for limited sample and single cells at faster throughput Hela Dilution experiments. 50 pg to 5 ng injected on column and analyzed by Orbitrap Astral MS by...
• Session:http://genome.ucsc.edu/s/nirfriedman/cfChIP-seq • Track hub:http://www.cs.huji.ac.il/~nir/Hubs/cfChIP-seq/hub.txt Additional data from public repositories are listed here: The datasets are as follows: UCSC known genes (AH5036); Ensembl transcripts (AH5046); genomic ann...
Mass spectrometry analysis was performed on protein lysates (Supplementary Data 4), and we found cell cycle proteins to be differentially expressed, as expected, among these populations, including mAG and GEMININ that were enriched in S/G2/M cells, and mKO2 and CDT1 that were enriched in ...
Chip assayhas already been widely applied to dynamic analysis of genes and proteins. The development ofmicro total analysis system(μ-TAS) in recent years provides excellent manipulation and detected platform for single-cell analysis; it makes fast complete manipulation and analysis of single-cell ava...
ChIP-seq data supporting the findings Deposited in GEO under accession code GSE160365, publicly available since 31 October 2023. Mass spectrometry data Deposited in ProteomeXchange with primary accession code PXD046270. Human PDAC data derived from TCGA Research Network Available at Human Protein Atlas...
To search for such driver-lncRNAs, we here perform a genome-wide analysis of fitness-altering single nucleotide variants (SNVs) across a cohort of 2583 primary and 3527 metastatic tumours. The resulting 54 mutated and positively-selected lncRNAs are significantly enriched for previously-reported ...
ChIP analysis of LSD1 occupancy on gene promoters in (D) D283Med and (E) D425Med cells expressing Flag‐KBTBD4WT or Flag-KBTBD4R313PRR upon doxycycline (1 µg/mL) treatment for 3 days. Data are expressed as percentage of input. F, G Dotplot of KEGG pathway enrichment analysis...