Cell adhesion experiments are important in tissue engineering and for testing new biologically active surfaces, prostheses, and medical devices. Additionally, the initial state of adhesion (referred to as nascent adhesion) plays a key role and is currently being intensively researched. A critical step...
4Test Cell Adhesion-Promoting Activity of Laminin Testing the capacity of laminin to mediate cell adhesion follows the same protocol as for testing cell adhesion-promoting activity of any other extracellular matrix proteins. An easycell adhesion assaywas described in details (Aumailley, Mann, von der...
Homogeneous cell adhesion Cell density dependent behavior Why is cell seeding a critical step in all cell culture experiments? Cell seeding is usually the first protocol step and a standard procedure in cell-based experiments. A correct and standardized cel...
1b). We speculated that the inefficient fluorescence labeling of GRAPHIC in cancer cells was due to the unstable cell–cell adhesion of cancer cells. To achieve efficient optical labeling of cell–cell interactions involving cancer cells, sGRAPHIC was conceived using a combination of GPI-anchored N...
our analysis predicted activation of inflammatory immune response in aCap by activation of several receptors involved in immune cell adhesion (Itgb1,Itga2,Icam1and2,Esam) with simultaneous upregulation of their ligands in stromal cells such asCol13+fibroblasts, myofibroblasts, Pericyte 1 (Tnc) and ...
4a). Among other adhesion molecules, notably integrins failed to co-localise (Supplementary Fig. 7b–d). However, the downstream effector of N-cadherin, α-catenin, did co-localise with fascia EPFs and N-cadherin in the scar area, consistent with a potential role for this molecule in ...
Developing programmable bacterial cell-cell adhesion is of significant interest due to its versatile applications. Current methods that rely on presenting cell adhesion molecules (CAMs) on bacterial surfaces are limited by the lack of a generalizable str
Fig. 1: Single-cell RNA-seq experiment of pancreatic tissue, taken from Ptf1a-CreER, LSL-Kras G12D, LSL-tdTomato mice at different time points post-tamoxifen injection (PTI). a–h The accumulation of duct-like structures and PanINs at different time points post-tamoxifen injection (PTI)....
Interactions of cells through secreted ligands and plasma membrane receptors, secreted enzymes, extracellular matrix proteins or cell–cell adhesion proteins and gap junctions. Cell-type deconvolution Decomposing the cell-type composition of individual barcode regions based on a reference data set to obtain...
Adipocyte nuclei were isolated following a modified nuclear isolation protocol60. In total, 50 mg of fresh or frozen WAT was first minced into 1–3 mm pieces and then homogenized on ice in 0.1% CHAPS in CST buffer supplemented with 0.2 U/μl RNAase inhibitor (RI) using a Dounce...