Reduced 还原IgG: 25 min, Non-reduced 未还原IgG: 35 min Detection Capability检测能力UV Absorbance at 220 nm 紫外吸收220nm Typical Voltage分离电压Separation: 5750 V Sample Injections per Cartridge每卡盒进样数 100 guaranteed, 200 maximum 确保100次,zei多200次 ...
The Maurice Turbo CE-SDS™ cartridge enables rapid CE-SDS analysis—5x faster—with high sample throughput. The cartridge lets you analyze reduced samples in 5.5 minutes and non-reduced samples in 8 minutes. This poster illustrates the reproducibility, linearity, dynamic range, and limit of detec...
The IgG Purity and Heterogeneity Assay provides high-resolution separation for either reduced or non-reduced IgG molecules. 关于CE-SDS 毛细管凝胶电泳应用的更多信息,请参见下面的应用通讯: “Assay of IgG Purity and Heterogeneity Using High-Redolution Sodium Dodecyl Sulfate Capillary Gel Electrophoresis” ...
[4]. A Series of Collaborations between Various Pharmaceutical Companies and Regulatory Authorities Concerning the Analysis of Biomolecules Using Capillary Electrophoresis.” Chromatographia 2006, 64, September(No. 5/6). [5]. The PA 800 Plus ensures requirements of 21CFR211.194 can be met by providi...
Analysis: of non-reduced and reduced monoclonal antibodies (mAbs) by capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) is routinely used to detect product size variants and process-related impurities. Levels of high molecular weight (HMW) forms obtained from this method usually trend comparably...
To improve capacity and streamline sample preparation, an automated robotic platform was developed using the PhyNexus Micro-Extractor Automated Instrument (MEA) for both the reduced and nonreduced CE-SDS assays. This automated sample preparation normalizes sample concentration, removes salts and other ...
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2D measurements of the light and heavy chain of the reduced NIST mAb with the 8-port valve and the optimized decomplexation strategy demonstrates the increased robustness of the system. The presented novel set-up is a step toward routine application of CE(SDS)-CZE-MS for impurity ...
They can hardly be reduced by regular method optimization such as changing buffer pH, denaturing temperature or incubation time to achieve the (LC + HC)% expectation. Here, the peaks are first characterized and determined to be non-covalently formed LC-LC dimers by multiple techniques including ...
Capillaries of the1D CE(SDS) separation were conditioned prior to each measurement by flushing with 0.1 M NaOH, 0.1 M HCl and water at 3 bar for 3 min. For the injection via valve experiments (no separation in the1D), the capillaries of the1D were flushed with reduced NIST ...