品系描述 在Cdx2基因exon 2两侧分别插入loxP位点。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cdx2基因的小鼠模型。 应用领域:肿瘤相关研究 *使用本品系发表的文献需注明: Cdx2-Flox mice (Cat. NO. NM-CKO-200036) were purchased from Shanghai Model Organisms Center, Inc.. ...
Cdx2 was specifically activated in adult HSC using the tamoxifen-inducible SclCreERT:Cdx2 model and compared with LysMCre:Cdx2 mice that selectively activated Cdx2 in the myeloid lineage, commencing at committed myeloid progenitors. SclCreERT:Cdx2 mice developed a lethal myelodysplastic phenotype ...
The targeting construct, the targeted allele and the expected Cre recombination product are shown below. E1, exon 1 of Cdx1; B, BamHI; K, KpnI; H, HindIII; P, PstI; X, XhoI. (B) Multi-enzyme Southern blot demonstrating the fidelity of targeting. To characterize the models used in ...
(b) Quantification of the surface of the Brunner’s glands in 3–4-month-old control jojo-Flag-miniCDX2 (ctrl, n=12) and jojo-Flag-miniCDX2::VilCre mice (miniCdx2, n=9); **P<0.01. (D) Pdx1 immunofluorescence staining in control jojo-Flag-miniCDX2 (ctrl) and jojo-Flag-miniCDX...
S Robine (CNRS, UMR 144, Institut Curie, Paris) for the VilCre mice, Professor B Chabot (Université de Sherbrooke) for the plasmids pHis-SRp30c and pHis-ASF/SF2, and Dr. JF Launay (Inserm U682, Strasbourg) for help in C2T antibody purification. The data described here have been ...
intestinal sections: nuclear immunostaining in the crypt epithelium of wild-type mice; loss of staining when C2T is preincubated with an excess of immunogenic peptide; absence of immunostaining in the intestinal epithelium of conditional knockout (KO) Cdx2f/f::AhCreERT mice.7 Bars: 50 μm. ...
Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。 查看 【小鼠大学问】基因工程小鼠的命名规则 常见的基因工程小鼠可以分为两种命名方式,包括基因定点修饰的小鼠命名,比如:敲除、敲入、点突变等...
Cre activation in CDX2P9.5-G19Cre;R26R mice.doi:10.1371/journal.pone.0132435.g004Sasada TatsunariHinoi TakaoSaito YasufumiAdachi TomohiroTakakura YujiKawaguchi YasuoSotomaru YusukeSentani KazuhiroOue NaohideYasui WataruPLOS ONE
Effects of CDX2 on prognosis and chemotherapy responsiveness in mismatch repair-deficient colorectal cancer: Effects of CDX2 in mismatch repair-deficient colorectal cancerdoi:10.1002/bjs5.91WileyBjs Open
Here, to unambiguously address at which developmental stage Cdx2 becomes first required, we genetically deleted Cdx2 from the oocyte stage using a Zp3-Cre/loxP strategy. Careful assessment of a large cohort of Cdx2 maternal-zygotic null embryos, all individually filmed, examined and genotyped, ...