虽然 Cyclin D1 在前列腺上皮细胞和间质细胞中均见表达, 但仅在 BPH 间质细胞中表达高于 NP 且与 PCNA 指数正相关, 说明 Cyclin D1 参与前列腺正常生理过程, 但主要促进间质细胞增殖、增生。 Cyclin E 主要分布于前列腺上皮细胞, 但 NP 与 BPH 组织中表达之间差别无显著性意义, 推测 Cyclin E 对前列...
CDK4/6与cyclinD结合,促使视网膜母细胞瘤蛋白(Rb)磷酸化,部分转录延展因子E2F的活性得以释放,用于合成大量的RNA与蛋白质,为染色体复制做准备。 G1后期,CDK2与cyclinE结合形成蛋白酶体复合物CDK2-cyclinE并活化,促使Rb进一步磷酸化,诱导转录因子E2F的持续表...
目的分析探讨人乳腺癌细胞中cyclinD1的表达受到反义RNA抑制后,细胞增殖能力的变化以及 cyclinE,CDK2和p21cip1(cip1/waf1/sdi1)基因表达受到的影响. 方法将表达cyclinD1反义RNA的重组质粒转入人乳腺癌细胞,由此抑制细胞中cyclinD1的表达,然后分析细胞生长速率和各时相细胞分布比例的 变化,并通过Northern blot分析有...
目的探讨补阳还五汤对局灶性脑缺血后抑郁大鼠海马区Cyclin D1、Cdk2的影响。方法采用大脑中动脉局灶性永久性线栓法结合慢性不可预见温和应激(chronic unpredictable mild stress,CUMS)及孤养法制备脑缺血后抑郁模型,将动物随机分为假性手术组、脑缺血组、卒中后抑郁组、脑卒中后抑郁+氟西汀组(以下简称氟西汀组)和...
结论 cyclinD1表达的抑制可明显减弱人乳腺癌细胞的异常增殖,同时表明同为细胞周期调控基因的cyclinE和CDK2的表达与cyclinD1的表达 密切相关,而p21cip1的表达不受cyclinD1的影响.doi:10.3321/j.issn:0529-1356.2000.03.008桑建利北京师范大学生命科学学院叶颖江北京医科大学第二附属医院VIP解剖学报桑建利,叶颖江,王永潮....
Instead, the researchers found that cyclin D1 or a related regulatory protein, cyclin D2, must first pair up with specific kinase enzymes -- called CDK 4 and CDK6 -- to drive myeloma cells toward division. "It's as if the cyclin molecule is the gas, and the enzyme is the engine. ...
研究发现hsa-circ-0000520缺失诱导P21和P27表达增加,细胞周期相关蛋白CyclinD1表达降低,凋亡相关蛋白Bax表达增加,Bcl-2表达减少。因此,沉默hsa- circ-0000520能够阻断宫颈癌细胞周期的进展。 生物信息学分析和双荧光素酶报告基因检测都揭示了hsa-circ-0000520与miR-1296之间的靶向关系,两者存在结合位点,过表达hsa-circ-...
Cyclin D1 is a key mediator of cell cycle progression in hepatocytes, and transient expression of this protein is sufficient to promote robust proliferation of these cells in vivo. In cdk2-/- mice and animals treated with the cdk2 inhibitor seliciclib, cyclin D1 failed to induce hepatocyte ...
Cyclin D1 is known as a promoting factor for cell growth. We previously showed, however, that the expression of cyclin D1 increases markedly in senescent human fibroblasts in vitro. Here we investigate whether the overexpression of cyclin D1 inhibits cell proliferation. Colony formation after trans...
This page is a summary of: Cdk2 plays a critical role in hepatocyte cell cycle progression and survival in the setting of cyclin D1 expression in vivo, Cell Cycle, September 2009, Taylor & Francis, DOI: 10.4161/cc.8.17.9465. You can read the full text:Read Contributors The following have...