EC50 values were determined by nonlinear regression and a four-parameter algorithm (GraphPad Prism 7). Clonogenic Assays 500 cells per well were plated in six-well dishes in triplicate. After overnight incubatio
ChIP-seq peaks were detected using a peak-finding algorithm, MACS version 1.4.2 (Zhang et al., 2008) with the default p value threshold of enrichment of 1 × 10−5 used for all data sets. Active enhancers, ranked according to the magnitude of the H3K27ac signal, were defined as ...
To simulate growth and mutation during passaging in drug, we used the Binomial-Negative Binomial (BNB) algorithm54. For resistant cells, which do not undergo further mutation, expansion is simulated for one time step Δt as the sum of m ~ B(n0, 1 − α) and n ~ NB(m, 1 − β...
EC50 was determined from the nonlinear regression and a four-parameter algorithm on the GraphPad Prism software. Immunofluorescence Cells were fixed with 4% PFA in PBS at room temperature, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in PBS at room temperature. Cells ...