In fact, CDDP given concurrently with RT leads to a potentiation of cell killing, based on an inhibition of the recovery of the radiation-induced damage and which is more evident in inherently sensitive cells to CDDP alone [1]. Furthermore, the simultaneous administration of RT and chemotherapy...
Continuous Infusion (CI) of cisplatin (CDDP) and concurrent radiotherapy (RT) in non resectable stage III lung cancerdoi:10.1016/0169-5002(91)91964-DRavasi, GBedini, AvMilani, FGramaglia, ATavecchio, LSpreafico, CMarchiano, ALung Cancer...
MHCIb,HLA-DR) ofSKo” /CDDP cel l streated wi thor wi thout CI K cel l s were detected by el ectronmi croscope,^斤rTand FCM.Thechangesof cyto喇ne(IL-2,TNF· d,IFN-T,GM—CSF) i nesra of SCID mi cebeari ngSK-oV3/CDDP cel l sweredetectedbyradi oi mmuni tandELl SA....
方法:对前期实验建立的耐顺铂(cisplatin,CDDP)细胞株OSC-19/CDDP,采用CCK-8法检测其对不同化疗药物的耐药性;RT-PCR检测亲代与耐药株的多药耐药基因1(multidrugresistance1,MDR1)表达。通过微阵列芯片筛查在OSC-19/CDDP与亲本细胞OSC-19中差异性表达的miRNAs,并通过RT-PCR进一步验证基因芯片的结果。结果:人舌癌多药...
确定每一组别的半数抑制浓度(IC50),再进一步计算出对照组实验数据与实验组的倍数关系即逆转倍数.②将细胞系分为对照组和实验组,实验组细胞系给予AE干预,用RT-PCR法检测各组polβ的表达水平.③所有实验数据一律选择SPSS17.0进行规范化处理,数据用x±S进行标注,IC50采用直线回归法进行计算,采用t检验法对比两组数据...
根据加与不加两组数据行直线回归统计计算出各组半数抑制浓度(IC50),计算逆转倍数=对照组IC50/试验组IC50.2,将细胞系分为实验组和对照组,实验组细胞系用藤梨根提取物干预,RT-PCR法分别检测以上两组细胞GST-π的表达.3,全部数据均用SPSS13.0统计软件包处理,数据以x±S表示,IC50计算采用直线回归,两组数据比较,...
多药耐药口腔腺样囊性癌细胞株(Acc-3／CDDP)的建立及其细胞中相关耐药基因表达的变化
Acc一3/CDDP,检测其多药耐药性、探讨与其亲代Ace一3细胞系的耐药相关基因变化。方法:用浓度递增法建立Acc一3/CDDP耐 药细胞系;用MTr法检测Ace一3/CDDP细胞的耐药指数,RT-PCR检测多药耐药基因MDR一1、MRP一1、GST一1T和Bel一2基因的 mRNA表达水平。结果:Acc一3/CDDP对CDDP、5一氟尿嘧啶(5～Fu)、长春新碱(...
According to a recent report, JMJD2A can promote CDDP resistance in ovarian cancer cells [16]. Here, we aimed to examine the effect of JMJD2C on the drug resistance of uveal melanoma cells. RT-qPCR results displayed that the expression of JMJD2C in uveal melanoma tissues was significantly...
表明一定浓度的 RAE 对人胃癌 SGC7901 细胞对顺铂的耐药可以起到部分逆转的作用.③RT-PCR法分别检测两组细胞 polβ的表达,对照组显示 polβ基因的表达明显增高,说明一定剂量的 RAE 能够降低 SGC7901/cDDPpolβ的基因表达水平,这一研究为藤梨根提取物抗肿瘤作用及其逆转耐药提供了实验方向,为进一步临床应用以及...