We demonstrate that the C-terminus of OsCDC48 is essential for maintaining its full ATPase activity and OsCDC48/48E interaction is required to modulate cel
The E. coli spreading through the states is "toxin-producing," the CDC states — specifically a toxin known as Shiga. People get sick within two to eight days of swallowing the germ, which causes diarrhea, stomach cramps and vomiting. Although most recover in one week, it could l...
coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [33]. His6-T7-Net1(1-341) (Net1N, RDB1153) [11] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28Kix1...
aH&E-stained heart sections from MCM control andClk4-cKO hearts. Scale bar: 1 mm.b,cSummary data for LV internal diameter (LVID) and interventricular septum (IVS) thickness.d,eWGA staining and quantification of cardiomyocyte cross-sectional area. Scale bar: 50 μm.f,gWGA staining, a...
Target shRNA sequences were inserted into BR-V-108 vectors, and then transformed into E. coli competent cells (Tiangen) for plasmid extraction using EndoFree maxi plasmid kit (Tiangen). Qualified plasmids were packaged for lentivirus production using 293T cells which were co-transfected with BR-...
E. coli Rosetta 2 (DE3) T1R Novagen N/A Deposited Data H. sapiens p97-ND1:p47 complex structure (Dreveny et al., 2004) PDB: 1S3S H. sapiens p97-ND1:ASPL complex structure (Arumughan et al., 2016) PDB: 5IFS A. thaliana CDC48-ND1 structure This paper PDB: 6HD0 H. sapiens/...
Cdc25s Phosphatase Inhibitory Activity The selected compounds, whose structures and purity consistency were checked by HMRS analysis (Supporting Information S8), were tested in a dose-response assay on Cdc25 phosphatases. Eight of the twelve tested compounds (798827, B8063, E7887, F5312, J3955,...
The CDC declared the E coli outbreak linked to McDonald's Quarter Pounders over after 104 cases across 14 states and at least one death.
AUGUSTA, Maine -- The E. coli infection that killed one Mainetoddler and hospitalized another in...Farwell, Jackie
63.HoweCW.ExperimentalwoundsepsisfromtransientEscherichiacoliradicalmastectomycomparedwithtylectomywithaxillarylymphnode bacteremia.Surgery1969;66:570-4.dissection.AmJSurg1991;161(5):584-8. 64.VelascoE,ThulerLC,MartinsCA,DiasLM,ConalvesVM.Riskfactors91.HolleyDT,ToursarkissianB,VansconezHC,WellsMD,KenadyDE...