This clarification of the mechanisms underlying the CD8 + CD28 − expansion in HIV-induced pathogenesis may have important therapeutic implications.Dalod, MarcLaboratoire d'Immunologie des pathologies infectieuses et tumorales, Unité 445 INSERM, Institut Cochin de Génétique Moléculaire...
Altered ex vivo balance between CD28+ and CD28− cells within HIV‐specific CD8+ T cells of HIV‐seropositive patients 来自 Wiley 喜欢 0 阅读量: 20 作者:M Dalod,M Sinet,Deschemin, JeanChristophe,S Fiorentino,A Venet,Guillet, JeanGérard ...
FMO, fluorescence minus one control. Additional file 8: Figure S3. The T-cell differentiation stage impacts the circulating CD28/PD-1 subset distribution and changes as a function of the activation interval. A Unstimulated ex vivo PBMC from NSCLC (n = 22), melanoma (n = 10), ...
T cells from healthy donor peripheral blood mononuclear cells were purified by negative selection using a T cell isolation kit (Miltenyi) and subsequently activated with CD3/CD28 Dynabeads (Thermo Fisher Scientific) at a cell-to-bead ratio of 1:3. A lentiviral vector carrying a previously ...
11). Gating was confirmed with fluorescence-minus-one (FMO) controls for low-density antigens. RIP-seq RIP-seq was conducted following a previously reported protocol with minor modifications26. Mouse activated CD8 T cells (anti-CD3/CD28, 5 μg/ml, 24 h) or Jurkat-YTHDF2 OE cells ...
Intracellular cytokine staining assays were performed as previously described38 with 4 μM peptide, anti-CD28 and anti-CD49d MAbs (1 μg/mL each; Becton Dickinson) and brefeldin A (Sigma, St Louis, Missouri)/mL. After a 6-hour incubation cells were washed and stained with the surface anti...
The majority of immune-oncology assays use pre-stimulated effector T cells with anti-CD3 and anti-CD28 antibodies, relatively high doses of cytokines (e.g. IL-2, IFNγ) or unspecific stimulation with concanavalin A or PMA and ionomycin, which could bias the results when testing immunotherapeuti...
Indeed, scRNA-seq revealed that silencing the top predicted positive regulators of memory (DNMT1, FOXO1 and MYB) led to decreased expression of CCR7 and other memory-associated genes (such as IL7R, SELL, CD27, CD28 and TCF7) and increased expression of effector-associated genes (GZMA, GZMB...
As described previously2, cells isolated from chronic infection have a distinct transcriptional profile compared to the ones isolated from an acute infection (Fig.1a). Upon in vitro restimulation in presence anti-CD3 and anti-CD28 antibodies, there were significant changes in CD8 T cells isolated ...
The pretreated or non-pretreated MC38 cells were co-cultured with CD8+ T cells at the ratio of 1:1 in the presence of CD3/CD28 and IL-7 for 2, 24, and 48 h, respectively. After 24 h of co-culture, APS treatment with 500 μg/mL significantly suppressed the high expression ...