弱激动剂抗体降低毒性,但显示有限的T细胞活化。 2024年12月2日,清华大学傅阳心、中国科学院生物物理研究所彭华共同通讯在NatureBiomedical Engineering(IF=26.8)在线发表题为“Concurrent intratumoural Treg cell depletion and CD8+ T cell expansionviaa cleavable anti-4-1BB–interleukin-15 fusion protein”的研究论...
To address this question, specifically antibody-mediated cell depletion against CD8, CD4 T or B cells respectively was performed in aged mice followed by a sciatic nerve crush for 3 days. Interestingly, CD8 monoclonal antibody promoted nerve regeneration while depletion of CD4+ T cells or B cells...
We investigated the mechanisms by which CD8 + T-cell trafficking in placenta contributes to perinatal brain injury by studying effects of maternal CD8 + T-cell depletion (DEP) in a mouse model of intrauterine inflammation (IUI). Maternal CD8 + T cells were depleted with anti-CD8 + antibodies...
in vivo CD8+ T cell depletion Walsh, K. B., et al. (2014). “Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy.” J Virol 88(11): 6281-6293 n vivo CD8+ T cell depletion...
potentially, fumarate depletion could be an important strategy for tumor immunotherapy. 微环境中的代谢改变显着调节肿瘤免疫敏感性,但潜在机制仍不清楚。 在这里,我们报告富马酸水合酶(FH) 耗尽的肿瘤表现出功能性 CD8 + T 细胞活化、扩增和功效的抑制,以及增强的恶性增殖能力。 从机制上讲,肿瘤细胞中的 FH ...
Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques. J. Exp. Med. 189, 991–998 (1999). CAS PubMed PubMed Central Google Scholar Pircher, H. et al. Viral escape by selection of cytotoxic T cell-resistant virus variants in vivo....
应用 in vivo CD8+ T cell depletion/Western blot 产品形式 PBS, pH 7.0 Contains no stabilizers or preservatives 内毒素水平 <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定 纯度 >95% Determined by SDS-PAGE 无菌处理 0.2 μM filtered 生产形式 从组织培养上清液中纯化得到。 纯化形式 ...
Peripheral Blood Stem Cell TransplantationTransplantation, HomologousWhole-Body IrradiationPrior studies suggest that depletion of CD8+ T cells from donor bone marrow or donor lymphocyte infusions can reduce graft-versus-host disease (GVHD) without compromising graft-versus-leukemia. We explored CD8 ...
Control mice were injected with 500µg ratanti-Trinitrophenol (clone 2A3, BioXCell). Antibodies were purified fromhybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma).Injection of the respective antibody resulted in depletion of >90% of CD4 orCD8 T cells for at least ...
本方案旨在通过去除 B 细胞、NK 细胞、单核细胞、树突状细胞、CD4+T 细胞、粒细胞,从外周血单核细胞 (PBMC) 中分离未接触的人 CD8+T 细胞。分离的 CD8+T 细胞无微珠和抗体,适用于任何下游应用。 分离原理 向起始样本中...