“variant humanized anti-CD70 antibody” are used herein in the broadest sense and specifically encompass full-length and native antibodies, monoclonal antibodies (including full-length monoclonal antibodies), polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), and antibody or ...
blocking buffer for 1 hour at 24°C. The primary antibody was diluted 1/100 in TNB blocking buffer and incubated with the sample for 24 hours at 4°C. An HRP Polymer-conjugated Goat anti-Rat polyclonal was used undiluted as the secondary antibody. Detected using a Streptavidin-Alexa 546 ...
from receiving a signal through CD27 during priming, subsequently exhibit a specific defect in their capacity for secondary expansion (Figs 1,2,3,4). This outcome is seen with both the endogenous polyclonal repertoire as well as with monoclonal transgenic CTL in single- and double-transfer settin...