in Antibody Therapy FCγ receptors (FCγRIII/CD16,FCγRII/CD32, andFCγRI/CD64) are cellular receptors for IgG that contribute to antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and immune complex clearance, and comprise several subtypes (Table 1). FCγR polymorphisms can...
Mechanistic studies demonstrated that CD64 or its intracellularly truncated analog CD64t effectively capture monomeric IgG and occupy their F, and the IgG bind and occupy their target antigens. In three applications of the approach, human CD64t-engineered thyroid epithelial cells, pancreatic beta ...
the rhesus D blood type antigen, ADCC and CDC assays were performed with a humanized IgG1 anti-Rh(D) antibody (Supplementary Fig.4a,b). Whereas HIP iECs were killed in both assays, HIP iECsCD64were resistant against anti-Rh(D) cytotoxicity. We next tested the binding affinities of IgG ...
Regarding the two patients with prostate cancer, one has had a 90% reduction in serum prostate-specific antigen (PSA; 118–11 ng/ml), which has persisted for several months; the other patient with prostate has had a 70% reduction of serum PSA (872 ng/ml to 208 ng/ml) within the ...
Antigen targeting to myeloid-specific human Fc gamma RI/CD64 triggers enhanced antibody responses in transgenic mice Receptors for the Fc region of IgG (Fc γ Rs) are members of the Ig superfamily that function in t... Heijnen,V Vugt,Fanger,... 被引量: 0发表: 0年 IFN‐γ induces the...
Neutrophilic granulocytes express their Fcgamma receptor, also known as the CD64 antigen, predominantly when they are activated. This makes neutrophil CD64... JJML Hoffmann - 《Biochemia Medica》 被引量: 69发表: 2011年 Human monocytes expressing a CEA-specific chimeric CD64 receptor specifically ...
) one of three additional criteria (positive CSF culture, positive Gram stain, or positive bacterial antigen in the CSF). A child with the first three criteria was considered to have probable bacterial meningitis; a child with all four criteria was considered to have confirmed bacterial meningitis...
TO assess the functional role of FCγRI more directly, bispecific antibody 520C9 × 22 [F(ab')2 X F(ab')2] with specificities against both the tumor-associated antigen erbB2/HER-2 and FcγRI was used. Ovarian carcinoma cells (SK-ov3), overexpressing erbB2/ HER-2, were used as ...
Specific cell-surface antigens are expressed in large quantities after the leukocytes are acti- vated by bacteria or toxins (18, 19). Multicolor flow cytometric analysis also has the advantage over immunoassay of being able to localize the activated markers on a specific cell type, and the ...
Potentiation of lysis of leukaemia cells by a bispecific antibody to CD33 and CD16 (FcγRIII) expressed by human natural killer (NK) cells Bispecific antibodies recognizing tumour-associated antigens and trigger molecules expressed on immune effector cells have been shown to redirect cytotoxic... ...