NK, natural killerPCR, polymerase chain reactionPE, phycoerythrinPh, PhiladelphiaPPO, platelet peroxidaseThe MZ93 cell line, established from a patient with CML, expressed CD4, CD7, CD13, CD25, CD33, CD34, CD56
在细胞因子表达上,与 CD56dimCD16+ NK 相比,CD56-CD16+NK存在IL18受体基因(IL18RAP 和 IL18R1)、细胞毒性基因如 KLRF1 (NKp80) 和 NCR1 (NKp46) 以及抑制性 HAVCR2 (TIM-3) 显著下调。 这些数据均证实 CD56-CD16+ 细胞是真正的NK细胞,但它...
并且与 CD56dimCD16+ NK 细胞亚群相比,CD56-CD16+存在低亲和力 Fcγ 受体 CD16、中等亲和力 Fcγ 受体 CD32 和穿孔素2 基因的上调。 在细胞因子表达上,与 CD56dimCD16+ NK 相比,CD56-CD16+NK存在IL18受体基因(IL18RAP 和 IL18R1)、细胞毒性基因如 KLRF1 (NKp80) 和 NCR1 (NKp46) 以及抑制性 HAV...
NK细胞的杀伤作用不仅需要通过抑制性受体检测转化细胞上的MHC-I分子,还需要通过激活性受体激活NK细胞。自然细胞毒性受体(NCR)是一组自然杀伤细胞表面激活性受体,包括NKp46、NKp30和NKp44。 CD16( 或FcγRIII )也是一种激活性受体,主要由CD56dim NK细胞亚群表达,对IgG包被靶细胞的抗体依赖性细胞毒性( ADCC )至关...
Moreover, we demonstrate that blocking NK receptors NKp44 and NKp46 but not NKp30 could abrogate the regulation of proliferation associated with low‐dose IL‐2. The increase in NK cells was also accompanied by an increase in Treg cells, which is dependent on the presence of CD56bright NK ...
In contrast, no reactivity was observed with CD16-, CD158b-, or NKp46-specific antibodies (data not shown). Normal human lymph nodes were also studied using in situ RT-PCR to detect cells that express CD56 transcript (Figure1B). In 4 of 4 lymph nodes tested, a discrete number of ...
TheidentificationofepitoperecognizedbymonoclonalantibodyagainstCD56protein 体,降低 NK 细胞活性和抑制其杀伤靶细胞的能力[14-15].NK 细胞还可以通过血凝素 (HA)和 NKp44/NKp46 之间的相互作用有效地限制流感病毒(influenzavirus,IV)的复制和传播 .[16] 以上结 果表 明,NK 细胞在机体抵抗病毒感染过程中发挥 重要...
(NKp30, NKp44 and NKp46) and the secretion of granzyme K, resulting in mitochondrial dysfunction in activated T cells [5,62]. Furthermore, studies suggest that activated CD56brightNK cells in the lymph nodes may kill immature dendritic cells expressing low amounts of HLA-I molecules via ...
NK-92 cell line is highly cytotoxic; it expresses high levels of cytolytic pathway molecules, such as perforin and granzyme B29,30, lacks almost all killer cell immunoglobulin-like receptors (KIRs) and expresses major activating receptors (such as NKG2D, NKp30, NKp46)29. NK-92 cytotoxic ...
NK cells can recognize cells via two different mechanisms: (1) the so-called induced- self-recognition: activating receptors expressed on the NK cells (such as NKG2D and natural cytotoxicity recep- tors (NCRs) NKp30, NKp44 and NKp46) bind to stress- induced self-molecules that are usually...