NK, natural killerPCR, polymerase chain reactionPE, phycoerythrinPh, PhiladelphiaPPO, platelet peroxidaseThe MZ93 cell line, established from a patient with CML, expressed CD4, CD7, CD13, CD25, CD33, CD34, CD56 and NKp46. The additional karyotype abnormality of the Ph-positive leukemia cells ...
在细胞因子表达上,与 CD56dimCD16+ NK 相比,CD56-CD16+NK存在IL18受体基因(IL18RAP 和 IL18R1)、细胞毒性基因如 KLRF1 (NKp80) 和 NCR1 (NKp46) 以及抑制性 HAVCR2 (TIM-3) 显著下调。 这些数据均证实 CD56-CD16+ 细胞是真正的NK细胞,但它...
自然细胞毒性受体(NCR)是一组自然杀伤细胞表面激活性受体,包括NKp46、NKp30和NKp44。 CD16( 或FcγRIII )也是一种激活性受体,主要由CD56dim NK细胞亚群表达,对IgG包被靶细胞的抗体依赖性细胞毒性( ADCC )至关重要。 肿瘤通过建立免疫抑制肿瘤微环境来逃避免疫系统。涉及NK细胞的免疫逃避包括多种机制,肿瘤 利用NK...
并且与 CD56dimCD16+ NK 细胞亚群相比,CD56-CD16+存在低亲和力 Fcγ 受体 CD16、中等亲和力 Fcγ 受体 CD32 和穿孔素2 基因的上调。 在细胞因子表达上,与 CD56dimCD16+ NK 相比,CD56-CD16+NK存在IL18受体基因(IL18RAP 和 IL18R1)、细胞毒性基因如 KLRF1 (NKp80) 和 NCR1 (NKp46) 以及抑制性 HAV...
Moreover, we demonstrate that blocking NK receptors NKp44 and NKp46 but not NKp30 could abrogate the regulation of proliferation associated with low‐dose IL‐2. The increase in NK cells was also accompanied by an increase in Treg cells, which is dependent on the presence of CD56bright NK ...
(NKp30, NKp44 and NKp46) and the secretion of granzyme K, resulting in mitochondrial dysfunction in activated T cells [5,62]. Furthermore, studies suggest that activated CD56brightNK cells in the lymph nodes may kill immature dendritic cells expressing low amounts of HLA-I molecules via ...
In contrast, no reactivity was observed with CD16-, CD158b-, or NKp46-specific antibodies (data not shown). Normal human lymph nodes were also studied using in situ RT-PCR to detect cells that express CD56 transcript (Figure1B). In 4 of 4 lymph nodes tested, a discrete number of ...
Expression of NKG2D and NKp46 receptors on NK cell subsets (CD56bri, CD56neg and CD56dim), was comparable among ‘suboptimal’ and ‘super-optimal’ immune responders. Conclusions The pro-inflammatory CD56++CD16-- NK cells were higher among ‘suboptimal’ responders relative to ‘super-optimal’...
通过qmsp来评估来自122名hnscc患者和122名对照的存档全血dna中的nk细胞水平。焦磷酸测序和qmsp证实,nkp46中的去甲基化dna区将nk细胞与其他白细胞区分开来,并作为定量的nk细胞标志物。 鉴于以上所述,本发明的一个目的是提供一种基于dna甲基化分析的改进的、特别是稳健的方法作为优越的工具,以更方便且可靠地检测、...
方法采用流式细胞术检测33例慢性HCV感染者及21例健康对照者外周血CD56+T细胞的频数和细胞表面活化性受体NKG2C、CD+6、NKp46和抑制性受体CD+58a、NKG2A的表达水平;检测体外未刺激及K562细胞刺激作用下CD56+T细胞毒效应(CD+07a)和细胞因子分泌水平(IFN-γ和TNF-α),并分析上述3种CD56+T细胞功能指标之间的关联性。