The PB vs. CB CD56 bright had significant increased expression of KIR2DL2 (20.31 + 2.27 vs. 9.43 + 0.82, p 2 fold between CB and PB CD56 dim NK cells. The functional categories of differentially expressed proteins were binding (46%), catalytic (17%), signaling (15%), transcription (...
In this study we present new differential characteristics of NK cells expressing CD56 surface antigen in low (CD56dim) or high (CD56bright) density. In contrast to CD56bright NK cells CD56dim cells express killer cell immunoglobulin (Ig)-like receptors (KIR) such as CD158a, CD158b, and NKB...
但近年越来越多的研究表明CD56 brightCD16 dimNK细胞亚型在肿瘤的生物学作用中扮演"帮凶"角色,现将近年关于CD56 brightCD16 dimNK细胞在在肿瘤中发挥的效应综述如下。 关键词: NK细胞;TINK;TANK;肿瘤;进展 ABSTRACT Natural Killer (NK) cells can directly kill target cells without pre-sensitization of ...
has been described also in NK cells [4,47]. However, there are no data about the expression of cellular protective proteins in two subpopulations of NK cells, i.e. CD56dim and CD56bright cells during ageing. Therefore, the aim of our study was to analyze the expression...
2.3. Isolation of NK cells NK cells were negatively isolated from fresh or frozen PBMCs (Miltenyi human NK cells isolation kit); NK cells were divided into 7-AADnegative/CD3-CD56bright and 7-AADnegativeCD3-CD56dim NK cells by cell sorting. We chose to sort NK cell subsets based on the ...
In contrast to CD56bright NK cells CD56dim cells express killer cell immunoglobulin (Ig)-like receptors (KIR) such as CD158a, CD158b, and NKB1. However, c-type lectin-like receptors (KLR) CD94/NKG2 and CD161 are present on both subsets. The ability to form conjugates with susceptible ...
The frequency of the cytotoxic CD56dim NK cells did not differ between the two groups. Correlation analyses demonstrated a significant positive association of CD56bright NK cells with atheroburden (r=0.43; p=0.04), stenosis (r=0.58; p=0.005), plaque necrotic (r=0.71; p=0.002), calcium ...
NK activity was tested against K562 targets in 4-h 51Cr-release assays. The ratio of CD56dim/CD56bright NK cells was significantly different in patients vs. controls (10 vs. 16; p<0.01). A significantly greater percentage of CD56dim NK cells bound Anx in HNC patients (27±17%, median ...
Natural killer (NK) cells are lymphocytes of the innate immune system that, following differentiation from CD56bright to CD56dim cells, have been thought to retain fixed functional and phenotypic properties throughout their lifespan. In contrast to this notion, we here show that CD56dim NK cells...
(B) Representative histogram analysis of the CD56dim and CD56bright populations before (left panel) and after (right panel) expansion Throughout the culture period, fluorescence intensity of the CD56+ population became increasingly bright (Fig. 2B). Whereas on day 0, 44.0% ± 21.0% of the ...