CD45RO、CCR7、CD62L荧光抗体染色,用流式细胞仪进行检测各表面标志的表达情况。 结果: 1、乙肝肝癌组、乙肝癌旁组及对照组CD4 + T细胞占浸润淋巴细胞的比例分别为 (21.38±3.72)%、(10.12±2.42)%、(4.14±4.34)%。乙肝肝癌组显著高于乙肝癌旁组及 ...
Peripheral blood mononuclear cells were isolated, stained with labelled monoclonal antibodies specific for CD8, CD45RO, CD45RA, CD62L, CD27, TCR-ζ as well as isotype controls and examined by multicolour flow cytometry. Annexin V binding to CD8[sup +] T cells and PMA/ionomycin-induced IFN-...