The CD4 was initially identified by flow cytometry and then confirmed by immunohistochemistry. CD4 is a representative marker for helper T-lymphocytes and is present on a subset of thymocytes, peripheral T cells and monocytes or macrophages. Unlike CD2 and CD5, no B cell fractions are known to...
3.“差异”与“富集”两个工具、以及 4.两个映射工具这四个初级部分的学习后,我们将正式进入中级篇,第一讲的主题是亚细胞分群。 严格来说,亚细胞分群其实更适合作为高级篇的内容,因为它涉及多个关键因素,包括基础生物学背景、技术流程熟练度,以及最新的研究进展。不少从事医学与生信交叉研究的老师也坦言,分群过程...
Graphical Abstract 首先就揭示了该研究的核心内容:CD4+T细胞在早产儿肠道中的双面性 一面“天使”:多项研究表明CD4+Tem细胞在早期可以促进肠道发育,干细胞增殖。 一面“魔鬼”:CD4+Tem细胞产生的TNF-α介导肠道炎症,导致坏死性小肠结肠炎产生。 主要结果 胚胎期肠道上皮含有CD4+T细胞 首先通过流式检测妊娠13周至20...
But, since the target antibody is still attached to the cell surface, we recommend that you use an antibody that binds at a different epitope of the same surface marker. In the product insert, we give recommendations for the best suitable antibodies for use in FACS.Find additional tips, ...
ratio (IFN- gamma as a marker of Th1 cells, IL-4 as a marker of Th2 cells) was detected by flow cytometry: (a) HucMSC-EVs inhibited the proliferation of CD4 + T lymphocyte subsets; (b) HucMSC-EVs decreased the proportion of IFN- gamma ; and (c) HucMSC-EVs increased th...View...
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We also performed adoptive transfer with DCs harvested from female Pep Boy mice, which possess the differential pan-leukocyte marker CD45.1, to allow for tracking of DCs following PLND. CD11c+ DCs were similarly isolated using magnetic beads per the manufacturer’s recommendations (#130-052-001; ...
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 cons...
d, DMSO- or FUTi-treated WM793 cells immunofluorescently stained for endogenous HLA-DRB1 (green), KDEL (ER marker; red) and 4,6-diamidino-2-phenylindole (DAPI) (blue) (×20 magnification). e, Flow cytometric analysis for relative (rel.) cell surface fucosylation (top) and cell surface...
To do this, the software was instructed to consider Dynabeads as a cell marker and was provided with a Dynabead-only control in addition to the other unmixing controls. All processed data files were analyzed in FlowJo™ (BD Biosciences). For the CD4 T-cell experiment, initial purity and ...