However, in patients with a high number of CD34-positive cells, the FC method seemed to result in a higher percentage of positive cells compared to the IH method. In normal bone marrow, the ratio between the percentage of CD34-positive cells and the percentage of bone marrow blasts was ap...
Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to B lymphocyte precursors and CD34+ megakaryocytes. ...
CR was defined as bone marrow blasts ≤5%, no circulating blasts, with complete blood count recovery (neutrophil count ≥1000/μL and platelet count ≥100 000/μL). Partial remission required all the hematologic values of CR with a decrease of ≥50% in the percentage of bone marrow blasts...
Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. However, counting CD34+mononuclear cells may overestimate myeloid blasts in bone marrow smears due to B lymphocyte precursors and CD34+megakaryocytes. ...
Total RNA was extracted using RNAeasy Micro Kit (Qiagen, Hilden, Germany) from different cells: sorted CD34 þ from HIV patients and CD34 þ from UCMC, NK-cell populations, PHA blasts, PBMC and K562-cell line. Oligo(dT)-primed complementary DNA (cDNA) was prepared by standard technique...
a higher percentage of CD25-positive AML blasts have pooroverall survivalor relapse-free survival[126,127]. The enhanced CD25 expression in AMLs seems to be induced bySTAT5and MYChyperactivationoccurring in many AMLs: both STAT5 and MYC play a key role in leukemia-initiating activity[128,129]...
Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones and CD34+ megakaryocytes. ...
In RAEB, however, CD95/CD95L expression correlated with CD34+ cells but not with the percentage of BM blasts. After classification by the WHO proposal, the patients with refractory cytopenias with multilineage dysplasia showed features intermediary between RA and RAEB. No significant correlation was...
The CD4+ helper cells in the bone marrow were found positive for HIV-1 DNA in all patients. In contrast, CD34+ progenitor cells were positive in only one patient. Two monocyte samples and two samples of CD4-/CD34- lymphocytes/blasts (mainly B and CD8 lymphocytes) were positive. Proviral ...
Analysis of CD34-positive cells in bone marrow from patients with myelodysplastic syndromes and acute myeloid leukemia and in normal individuals: a comparison between FACS analysis and immunohistochemistry. Eur J Haematol, 56 (1996), pp. 124-129 CrossrefView in ScopusGoogle Scholar 25 K Ogata, Y...