%2,%,7%为CD62L。哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,.%7,2%,4%为CD62L。%,%,%。结论:小鼠脾脏淋巴细胞体外培养后,细胞表面标记物CD44和CD62L发生巨大变化C,D62L呈现明显下调趋势接,近消失,CD44也有下调。[关键词]表面标记物;小鼠;淋巴细胞ChangesofscuerlflacemarkersCD3,CD4,CD44,CD62Lonmouse...
方法:用淋巴细胞分离液分离小鼠脾脏淋巴细胞,37℃细胞孵箱中体外培养3 d后,向细胞中加入流式抗体Mouse CD3e PE-CY7、Mouse CD4FITC、Mouse CD44 PE、Mouse CD62L APC,同时设阴性对照,应用流式细胞仪检测细胞表面标记物CD3、CD4、CD44、CD62L的水平。结果:小鼠脾脏淋巴细胞分离后直接做流式检测,CD3~+CD4~+...
体外培养后 CD3+CD4+ 细胞占8畅96%,其中71畅82%为CD44+,11畅27%为CD62L+。 哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 20畅33%,其中 97畅72%的细胞为 CD44+,75畅74%为CD62L+。 体外培养后 CD3+CD4+ 细胞占7畅2%,CD44 阳性细胞占58畅21%,CD62L 阳性细胞仅占2畅77%。 结论:...
小鼠脾脏淋巴细胞体外培养前后细胞表面标记物CD3、CD4、CD44、CD62L的变化 Copyright©博看网 www.bookan.com.cn. All Rights Reserved.
The Tec-family kinase Itk plays an important role during T-cell activation and function, and controls also conventional versus innate-like T-cell development. We have characterized the transcriptome of Itk-deficient CD3+ T-cells, including CD4+ and CD8+
哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,CD3~+CD4~+细胞占20.33%,其中97.72%的细胞为CD44~+,75.74%为CD62L~+。体外培养后CD3~+CD4~+细胞占7.2%,CD44阳性细胞占58.21%,CD62L阳性细胞仅占2.77%。结论:小鼠脾脏淋巴细胞体外培养后,细胞表面标记物CD44和CD62L发生巨大变化,CD62L呈现明显下调趋势,接近消失,...
different treatment groups (Fig.5h, j). In untreated tumors, CD4 T cells and Tregsdominated the landscape, together with a naïve-like CD62L+CD44-Tcf1+CD8 T-cell population (Fig.5i, jand Supplementary Fig.7dMC10-01, -04, -05, -10 and Supplementary Fig.7e–gMC10-02, -03, -09...
对小鼠记忆T细胞的早期研究确定了CD62L+CD44+中枢记忆T(TCM)和CD62L–CD44+效应记忆T(TEM)细胞。TCM细胞的终末分化程度低于TEM细胞。单个原始T细胞和单个TCM细胞在抗原刺激下产生的后代大小和多样性非常相似。此外,来自单个初级TCM细...
Unlike the spleen and blood, the classic CD4(+) and CD8(+) T lymphocytes in the kidney had a high proportion of activated CD69(+) and effector/memory CD44CD62L ligand phenotypes. Also, a small percentage of CD4(+)CD25(+)forkhead box p3(+) and NKT cells was observed in perfused and...
Effector (CD44hi, CD62L-) and central (CD44hi, CD62L+) memory T cell subsets in mouse CD3e+ and either CD4+ or CD8a+ splenocytes. P Ethan,L Phoebe,L Feng,... 被引量: 0发表: 2015年 Analysis of intestinal lymphocytes in mouse colitis mediated by transfer of CD4+, CD45RBhigh ...