T细胞在胸腺中发育成熟,这些胸腺细胞群基于CD4和CD8的表达可区分为:CD4−CD8−双阴性(DN)胸腺细胞,CD4+CD8+双阳性(DP)胸腺细胞,未成熟单阳性(ISP)胸腺细胞,CD4+或CD8+单阳性(SP)胸腺细胞。 DN细胞可以根据CD25和CD44进一步分成4个亚群:DN1(CD44+CD25-)、D...
DN1阶段:最早的胸腺细胞,表现为CD44阳性,CD25阴性。 DN2阶段:开始表达CD25,仍为CD44阳性。 DN3阶段:在这一阶段,T细胞受体(TCR)β链基因开始重排。如果TCRβ链成功重排,细胞将继续发育。 DN4阶段:TCRβ链重排成功的细胞会继续增殖,失去CD44和CD25的表达,进入下一阶段。
Falk I, Eichmann K. Heterogeneity of the DN4 (CD44 – CD25 – ) subset of CD4 – CD8 – double negative thymocytes; dependence on CD3 signaling. Immunol. Lett. 2002; 82 :123–130. [ PubMed ]Falk I, Eichman K 2002. Heterogeneity of the DN4 (CD44- CD25-) subset of CD4-CD8- ...
Memory cells are typically CD44high,33 as were both CD25-CCR6+ and CD25+CCR6+ cells, whereas the respective CCR6- populations were CD44low. The same pattern was also detected for 2 other activation markers, CD11a (lymphocyte function-associated antigen 1 [LFA-1]) and CD54 (intercellular ...
Beta-selection: abundance of TCRbeta-/gammadelta- CD44- CD25- (DN4) cells in the foetal thymus. Eur J Immunol. 2007;37:487-500.Hager-Theodorides, A.L.; Rowbotham, N.J.; Outram, S.V.; Dessens, J.T.; Crompton, T. Beta-selection: abundance of TCRbeta´/gammadelta´ CD44´...
Pre-TCR/CD3 signals are essential for survival and maturation of (CD44–25+) DN3 thymocytes via the (CD44–25–) DN4 stage to CD4+CD8+ (DP) cells, a process termed β-selection. The exact developmental stages of apoptosis resulting from lack of pre-TCR/CD3 signals have so f...
Thymic immunophenotyping revealed significantly increased CD8 cytotoxic T cell, and CD25 both single positive population (p = .017鈥 0.010) and CD44CD25 double negative population (DN3) (p = .002) accompanied by an insignificantly reduced CD4 helper T cells (p = .451). Peripheral ...
The percentage of CD44high (memory) and CD45RBhigh (naive) cells was determined within the CD4+ T-cell and CD4+Foxp3+ T-cell population. Prior to aBMT, both in spleen and LNs, the ratio between memory and naive cells was dominated by naive cells (Figure 6A). Shortly after aBMT this ...
and Eichmann, K., Immature thymocytes that fail to express TCRbeta and/or TCRgamma delta proteins die by apoptotic cell death in the CD44(-)CD25(-) (DN4) subset. Eur. J. Immunol. 2001. 31: 3308-3317.Falk,I., Nerz,G., Haidl,I., Krotkova,A. and Eichmann,K. (2001) Imm...
Double-negative (DN) thymocyte subsets were examined in mice deficient in the CD3′ chain (ζ −/−). The HSA +CD44−CD25− subset was found to be missing, and DN thymocytes seemed to differentiate directly from HSA+CD25+CD44−cells to double-positive (DP) cells. When fetal ...