用于测量抗CD20抗体结合特异性的方法包括但不限于标准竟争结合测定法、用于监视B细胞的免疫球蛋白分泌的测定法、B细胞增殖测定法、Banchereau-样B细胞增殖测定法、用于抗体产生的T细胞辅助细胞测定法(Tcellhelperassaysforantibodyproduction)、B细胞增殖的共刺激观'J定法和用于B细胞激活标记的上调的测定法。参见,例如,...
Remarkably, transitional (CD24 CD38 ) B cells were virtually lacking in the LNs of rituximab-treated patients. Moreover, LN-derived B cells from ritux-imab-treated patients produced different amounts of various Ig-subclasses after anti-CD40/IL-21 stimulation ex vivo. Finally, afte...
FIG. 2 provides a schematic diagram of expression of various cell surface markers (CD43, IgM, IgD) during B cell differentiation and maturation. In the Tg+ mice, hCD20 is expressed on pre-B, immature B cells and mature B cells. FIG. 3 shows the result of screening Tg+ mice for huma...
Human tonsillar PCs (CD19+, CD38bright), CD4+ T cells and B cells (CD19+, CD38lo, IgD+) were sorted to more than 90% purity and cultured at 50 000 per cell type per round-bottomed well in 200 µL RPMI/10% FCS/penicillin G (200 U/mL). Transwells (0.2-µm pore size, ...
3.已知cd3许多年,因此其已经成为许多方面的目标对象。特别是,针对cd3或t-细胞受体复合物(其一部分是cd3)产生的抗体是已知的。已经描述了五种人源化okt3效应子功能变体抗体的体外特征(xu et al., 2000, cell immunol. 200(1):16-26)。 4.在发生1型糖尿病后在缺少持续的免疫抑制药物疗法的情况下,用抗-cd3...
91.权利要求86的方法,其中所述治疗剂选自抗CD25抗体、抗CD19抗体、抗CD21抗体、抗CD22抗体、抗CD37抗体、抗CD38抗体、抗IL6R抗体、抗IL8抗体、抗IL15抗体、抗IL15R抗体、抗CD4抗体、抗CDlla抗体、抗α-4/β-Ι整联蛋白(VLA4)抗体、CTLA4_Ig和抗C3b(i)抗体。 92.一种检测样品中⑶20抗原或表达⑶20的细...
前线NHL和进展性复发型NHL),自体干细胞移植后的NHL复发或自体干细胞移植难治性NHL(relapsing after or refractory to autologousstem cell transplantation),高级免疫母细胞性(high grade immunoblastic)NHL,高级淋巴母细胞性(high grade lymphoblastic)NHL,高级小非凹陷的细胞(high grade small non-cleaved cell)NHL,...