PE anti-human HLA-A,B,CAntibody和Biotin anti-human HLA-A,B,C Anti-body购于美国Biolegend公司; Alexa Flour ® 647AffiniPure Goat Anti-Mouse lgG,F(ab’)2 fragmentspecigic购于英国Jackson immuno公司; CellTrace™CFSE(Component A)和DMSO(...
生物素标记的单链 DNA 使用 Dynabeads™ MyOne™ Streptavidin T1(Thermo Fisher)进行富集,并与一个"桥接适配器"连接,该适配器设计用于实现目标片段的指数扩增。 随后在磁珠上进行巢式 PCR,并通过特定引物进行大小选择和扩增。 最后,DNA 文库在 MGI2000 平台上进行测序。 删除是指在切割位点处从 1 bp 到 100...
货号12-0191-82 CNY1,917.00 有货 查看价格 批量或定制请求 Application Created with Sketch. 图:1/1 CD19 Antibody (12-0191-82) in Flow Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or Anti-Mouse CD19 PE (filled histogram). Cells in the lymphocyte...
293freestyle悬浮细胞:(购自thermofisher公司)。 免疫球蛋白igg1恒定区fc段:(购自南京金斯瑞公司)。 bca法蛋白定量试剂盒:(购自pierce,货号为23252)。 cbs抗原固定溶液:将1.59gna2co3和2.93gnahco3溶于1l水中,调整ph值为9.6。 anti-humanfchrp二抗:(购自thermofisher公司)。 96孔底透板:(购自corning公司)。
PE 激发/发射光谱 565/576 nm 查看光谱 形式 Liquid 浓度 0.2 mg/mL 保存条件 4° C 运输条件 Wet ice RRID AB_2535464 靶标信息 CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B ce...
PE-labeled anti-CD45RA (Biolegend, Clone: HI100, Cat: 304107, Lot: B378521, 1:100 dilution) and PE-Cy7-labeled anti-CD62L (Biolegend, Clone: DREG-56, Cat: 304821, Lot: B373156, 1:100 dilution); CellTracker Deep Red (Thermo Fisher Scientific, Cat: C34565, Lot: 1987253, CTDR...
thermo vector 小鼠CD19分子试剂盒,进口试剂盒、**残留、病毒种类等化学试剂的盒子。一般医院、制药企业使用。核酸提取纯化类、蛋白检测类、RNA。 YAC-1细胞 vero细胞 U937细胞 U251细胞 T47D细胞 SH-SY5Y细胞 SF9细胞 RAW 264.7细胞 PC-12细胞 pc12细胞 MRC-5细胞 MCF-7细胞 L-02细胞...
将1×10 个HEK293FT细胞(Thermo Fisher)接种到T75烧瓶内并培养过夜, 然后使用CalPhos转染试剂盒(Takara, Mountain View, CA), 加入pPACKH1 Lentivector包装混合液(System Biosciences, Palo Alto, CA), 以及10μg的每种慢病毒载体进行转染。第二天用新鲜培养基替换培养基, 48小时后收集含有慢病毒的培养基。
DNA sequences were codon-optimized, synthesized by GeneArt (ThermoFisher, Regensburg, Germany), cloned into SFG [34], and verified by pyrosequencing (Epoch, Missouri City, TX). Retroviral transduction of cells T-cells, Daoy, Raji, or UPN03 were transduced with CAR, target, or eGFP.FF...
2的texmacs gmp无血清培养基(macs)重悬混匀,然后按照t细胞与活化磁珠cd3/cd28(thermo fisher)1:1的比例加入活化磁珠,放入37℃、5%co2细胞培养箱进行培养。 95.如无特殊说明,下述细胞培养的培养基均为含有200ui/ml il ‑ 2的texmacs gmp无血清培养基。