CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).CD16A is expressed by NK cells, macrophages, and a subset...
Crosslinking of CD16 in natural killer cells and monocytes results in activation of the MAPK and PI3K signaling pathways, thus regulating the production of TNF alpha, IL-6, and IL-1 beta.This monoclonal antibody has been shown to block Fc receptor functions.Applications Reported: This B73.1 (...
1). The known ligand binding site present in scavenger domain 3 is cleaved, thereby enabling CD163 cross-linking [10,41]. Proteolytic cleavage of CD163 is similar to that of TNF-α, in which is mediated by tumor necrosis factor (TNF)-α converting enzyme (TACE) or also known as a ...
The binding of antibody-antigen complexes to activatory and inhibitory FcγRs induces their cross-linking and subsequent signaling through immunoreceptor tyrosine-based activation motifs (ITAMs) and inhibition motifs (ITIMs), respectively. Cytoplasmic signaling includes an increase in intracellular calcium ...
Description: This B73.1 monoclonal antibody reacts with human CD16, a member of the Fc receptor family also known as Fc gamma RIIIa and Fc gamma RIIIb. This 50-65 kDa receptor is expressed on natural killer cells, neutrophils, monocytes, and macrophages. Crosslinking of CD16 in natural ...
Degranulation and cytokine production by NK cells in response to the cocrosslinking of CD16 and CD300a. Zebra plots showing the percentage of CD56 NK cells positive for CD107a, IFNγ, MIP-1β, and TNF from a representative untreated HIV-1 infected patient after stimulation with isotype ...
Again, CD16 cross-linking induced more interferon-γ transcription in NK cell clones than in non-MHC-restricted CTL clones. Also a higher tumor necrosis factor-α production of NK clones after CD16 cross-linking compared to CD16+dim CTL could be observed. These data suggest that after CD16...
It is capable of mediating antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis in response to crosslinking by antibody. The low affinity receptors for IgG, FcγRII and FcγRIII (CD16), are responsible for effector cell responses to immune complexes and represent the FcγRs ...
*Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8. [Abstract] *Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito...
We also measured cytokine (IL-2) secretion of Jurkat T-CD16A cells bound to BiKEs after cross-linking, which was achieved by either bivalent mouse anti-FLAG antibody or by 293T-gp160SCcells. Co-incubation of Jurkat T-CD16A cells with BiKEs opsonized 293T-gp160SCcells resulted in release...