CD14 MicroBeads UltraPure can be used for the positive selection or depletion of human monocytes and macrophages from cord blood or PBMCs. They are particularly suited to work with low quality starting material, i. e., older buffy coats. | Miltenyi Biote
Naive CD4+ T cells were obtained by negative selection using a naive CD4+ T-cell isolation kit (Miltenyi Biotec, Auburn, CA). Alternatively, naive CD4+ T cells were purified from umbilical cord blood mononuclear cells, using CD4 microbeads (Miltenyi Biotec), and used without altering the ...
Isolation of CD16À and CD16 þ monocyte subsets was performed using the CD16 monocyte isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions with some modifications. In brief, after magnetic depletion of NK cells and neutrophils, CD16 þ ...
, 2007). The cell suspension was cultured in complete medium for 48 hours in the absence or presence of IL-4 (25 ng ml-1), and adherent and non-adherent CD14+ cells were first positively enriched with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) before flow cytometric ...
Human CD14+-monocytes were isolated from PBMCs by anti-CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany), reaching 98% purity [26]. Autologous neutrophils were isolated to approximately 99.7% purity after dextran sedimentation of granulocytes and hypotonic lysis of erythrocytes, followed by...
2.2. Isolation and Culture of Peripheral Monocytes Monocytes were isolated directly from PBMCs using CD14+ MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer's instructions. We have previously confirmed, using flow cytometry, that the purity of the CD14+ cells after ...
2.3. Sorting of Different Cell Subsets to Detect the Infectivity for Adenovirus CD3+ T lymphocytes and CD19+ B lymphocytes were separated from PBMCs by magnetic bead-based cell sorting kit (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany), following the manufacturer's directions. In brief, ...