Mouse CD11c+ LP cells consist of more than three subsets distinguished by different expression of cell surface markers, such as CD11b, CD70, CX3CR1, and CD103; and it has been reported that each subset has a distinct mission (refs 3-7). This protocol details a method to isolate LP CD11c+ cells with high yield, viability and purity ...
英文名称CD11C cell flow cytometry analysis kit 英文同义词 CAS号 分子式 分子量0 EINECS号 相关类别 Mol文件Mol File 结构式 CD11C细胞流式仪分析试剂盒 性质 CD11C细胞流式仪分析试剂盒 用途与合成方法 安全信息 MSDS信息 CD11C细胞流式仪分析试剂盒 上下游产品信息 ...
其次,为了理解ABC在疫苗免疫模型中的功能,作者将B细胞特异Zeb2敲除的抗疟原虫抗原特异性B细胞(CD23cre/Zeb2fl/flIghg2A10B cell)转移至MD4小鼠,并通过辐照疟原虫疫苗免疫它们。正如预期,研究发现CD23cre/Zeb2fl/flIghg2A10B cell 不能形成ABC。然而,无论Zeb2缺失与否,小鼠的生发中心(germinal center,GC)B细胞和浆...
来自美国犹他大学的研究人员建立了特异性外泌体产生缺陷的小鼠品系和细胞,证明了CD11c+细胞中小GTPase Rab27A的缺失加剧了小鼠结肠炎,而DC细胞来源的外泌体miR-146a可以逆转这一炎症反应。相关研究以“CD11c+ myeloid cell exosomes reduce int...
小鼠CD11c+ 细胞分选试剂盒用于阳性分选小鼠脾细胞中的CD11c+细胞,其利用生物素化抗体标记目的细胞,再用Anti-Biotin超顺磁纳米微珠结合被标记的非目的细胞;当细胞悬液置于磁场中时,被磁珠结合的CD11c+ 细胞滞留在磁场中,非目的细胞悬浮在细胞悬液中保持游离,从而将小鼠脾脏或淋巴结中的CD11c+ 细胞分选出来。
感染后12,20和28天的免疫表征显示,B细胞特异Zeb2敲除小鼠中T滤泡辅助细胞(T follicular helper cell, Tfh)数量在第12天已经显著减少,然而GC B数量的下降和抗体亲和力降低在20/28天才被观察到。这些结果表明ABC在持续感染模型中能够促进Tfh...
CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression ...
In addition to CD11c+ DCs, there are CD11c− DC-like cells that express the T-cell co-receptor CD4. These CD4+ DCs are found in lymphoid tissue in the T zone (especially the outer T zone) but, unlike CD11c+ DCs, are also found in Bfollicles (Fig. 3). In humans, Flt3-ligand ...
固有层(LP)CD11c抗原递呈细胞,直接获取管腔内容物,并能直接控制CD4T细胞分化成Th1细胞,TH2或Th17细胞。小鼠LP CD11c 细胞由三个子类的细胞组成,区分于不同的细胞表面标志物,如CD11b,CD70,CX3CR1,CD103。据报道,每个子类都有其独特的使命。使用本方法分离LP CD11c 细胞,具有产量高,可行性好和纯度高的优点。
Together, our study identified pathological hallmarks of chronic EAE progression and suggests that CD11c+ microglia may regulate immune cell parenchymal infiltration in autoimmune demyelination. 关键词:CD11c+ microglia;EAE progression;CNS infiltration;p38α sexual dimorphism;Glia limitans...