Administration of DT at day 1 in DTR mice resulted in macrophage ablation at day 2–3 after injury with an increase in macrophages at day 4. Thus, the early recruitment of proinflammatory macrophages was critical for muscle regeneration; further studies are needed to define markers present on ...
thereby conferring selective sensitivity to diphtheria toxin (DTX) in cDCs. As expected, administration of DTX to theseZbtb46-DTRtransgenic mice significantly decreased the number of cDC1 and cDC2, and also decreased Ly-6C+HDE-induced APCs
CD11b-DTR mice receiving PBS served as a paired control. In control mice, LPS increased inflammatory vascular injury within 4 h as indicated by the enhanced generation of pro-inflammatory cytokines, such as interleukin (IL)-6, IL-1β, and IFN-β (Figures 1A–1C), neutrophil accumulation (...
Diphtheria toxin injection into CD11b DTR transgenic mice.MyungGyu, KimSun, Chul KimYoon, Sook KoHee, Young LeeSangKyung, JoWonyong, Cho
In ApoE/-CD11b-DTR-mice with a hypercholesterolemia-induced chronic endothelial injury transfusion of WT-derived CD11b+-monocytes stimulated by interferon-γ (IFNγ) decreased endothelial function, whereas interleukin-4-stimulated (IL4) monocytes had no detectable effect on vascular function. ...
Lung TNF-α levels were drastically reduced in CD11c-DTR BALB/c mice depleted of CD11c+ cells, and TNF-α-producing Ly6c(+)CD11b(+) cells were ... M Fei,S Bhatia,TB Oriss,... - 《Proceedings of the National Academy of Sciences》 被引量: 166发表: 2011年 Resident CD11b+Ly6C Lung...
We show that Clec9A–diphtheria toxin receptor (DTR) mice after depletion of CD103 þ CD11b À DCs developed severe, low-dose dextran sodium sulfate (DSS)-induced colitis, whereas the lack of CD103 þ CD11b þ DCs in Clec4a4-DTR mice did not exacerbate intestinal inflammation. The ...
Interestingly, the depletion of CD11b+ myleoid cells enhanced tumor growth as shown that tumor-bearing mice had shorter surviving days and larger tumor size after receiving two doses of DT injections compared to the control PBS group (22.4 days vs 25.0 days, respectively). This indicates that ...
Herein, we used transgenic mice expressing human diphtheria toxin receptor (DTR) under the control of Cd11b-promoter (DTR) mice to assess their specific role in regulating lung inflammatory injury. Administration of diphtheria toxin (DT) in DTR mice selectively depletes CD11b cells by causing ...
We show that Clec9A–diphtheria toxin receptor (DTR) mice after depletion of CD103+CD11bDCs developed severe, low-dose dextran sodium sulfate (DSS)-induced colitis, whereas the lack of CD103+CD11b+DCs in Clec4a4-DTR mice did not exacerbate intestinal inflammation. The CD103+CD11bDC subset ...