小鼠脂肪组织巨噬细胞同样可以用CD11b+、F4/80+作为标志物,但是F4/80mid群包含嗜酸性粒细胞 (Siglec-F+) 和中性粒细胞(GR1+),因此,圈门的时候要十分小心,只要圈下图中1门的位置,进一步可以通过CD11c+和CD301+/CD206+去区分M1...
Isolate highly purified mouse MDSCs (CD11b+Gr1+) from splenocytes, bone marrow, or peripheral blood in as little as 18 minutes with EasySep™.
Myeloid-derived suppressor cells (MDSCs) are a prominent component of the pro-tumoral response. The phenotype of and mechanisms used by MDSCs is heterogeneous and requires more precise characterization in gastric cancer (GC) patients. Here, we have identified a novel subset of CD45+CD33lowCD11b...
Findings demonstrated that the vast majority of CD45+ cells in triple negative breast cancer model are CD11b+ cells of the innate immune system. The CD11b+ cells in the tumor micro-environment of vehicle-treated mice were mostly comprised of GR1+ myeloid derived suppressor cells. On the other...
Recruitment of a myeloid cell subset (CD11b/Gr1 mid) via CCL2/CCR2 promotes the development of colorectal cancer liver metastasis. Hepatology 57, 829–839 (2013). 10. Diamond, M. S., Garcia-Aguilar, J., Bickford, J. K., Corbi, A. L. & Springer, T. A. The I domain is a ...
Cells were then labeled with indicated antibodies (as a cocktail: Anti-CD11b, anti-CD11c, anti-CD45 anti-Gr1 anti-Siglec F and anti-MHCII), for 30 minutes on ice. Samples were washed and analyzed using LSR-Fortessa (Beckman Coulter) and data were processed using Flow Jo software (...
Gr1+ Inflammatory Monocytes Are Required for Mucosal Resistance to the Pathogen Toxoplasma gondii 2008, Immunity Show abstract Toxoplasmosis - A global threat. Correlation of latent toxoplasmosis with specific disease burden in a set of 88 countries 2014, PLoS ONE Listeria monocytogenes: Epidemiology, hu...
1a–c). In contrast, flow cytometric analysis and immunohistochemical staining of Ly6G/Gr1, showed similar numbers of PMN associated with sub-epithelial space or lamina propria (LP)-enriched fraction from WT and Cd47−/− mice (Fig. 1c, d). We also determined numbers of PMN recruited ...